In vivo Magnetofection™

The main problems currently associated with systemic gene vector administration (gene therapy) include biodistribution of gene vector throughout the body, the lack of specificity towards a pathological site (bioavailability at the target site), the necessity of a large dose to achieve high local concentration, non-specific toxicity, inactivation of vectors due to undesired interactions with components of the in vivo milieu and other side effects due to high vector doses. Magnetofection™ resolves the problems related to diffusion limited process and to restricted bioavailability at the target site.

Principle

In vivo Magnetofection™ has been designed for in vivo targeted transfection and infection. This original system combines magnetic nanoparticles and nucleic acid vectors that will be retained after injection at the magnetically targeted site.
In this way, targeted delivery minimizes systemic distribution and reduces toxicity. Furthermore, the magnetic force will enhance the uptake of magnetic nanoparticles by the target tissue, and thus improve the efficiency of transfection or transduction.
This allows reducing the required nucleic acid or virus doses and the process time of delivery which is crucial for improvement of in vivo nucleic acid delivery.

What are the applications?

Three optimized In vivo Magnetofection™ reagents have been designed according to defined applications: In vivo PolyMag, In vivo DogtorMag, In vivo ViroMag, In vivo SilenceMag.
  • Non viral applications: In vivo PolyMag, a cationic polymer-based magnetic nanoparticles formulation, and In vivo DogtorMag, a cationic lipid-based magnetic nanoparticles formulation, have been designed for in vivo targeted transfection of various types of nucleic acids such as DNA, RNA and oligonucleotides.
  • Viral applications: In vivo ViroMag is an optimized nanoparticles formulation dedicated to viral vectors that allows reduction of titer virus. It is particularly suitable for Lentiviral/Retroviral, Adenoviral and Adeno-Associated Viral (AAV) vectors.
  • For gene silencing: In vivo SilenceMag is is a rapid, simple and highly efficient method dedicated to transfect small RNA (siRNA, miRNA) into target cells/tissue in vivo.

How do I use In vivo Magnetofection™ reagents?

Gene vectors /nanoparticles complexes can be easily administrated through various injection routes such as:
  • Systemic administration (intravenous, intra-artery),
  • Local administration (intratumoral, intracerebroventricular, intraperitoneal, intramuscular, subcutaneous).
Target tissue
Route of injection
Site of injection
Kind of magnet
Magnet position
Tumor
Intravenous,
Intratumoral
Tail vein
Tumor
All kind
External (subcutaneous tumor, brain tumor, well localized tumor)
Internal (interne organ tumor)
Endothelial cells
Intravenous,
Intra-arterial
Vessel of interest
Ear artery
Femoral artery
All kind
External (deep vessels)
Internal (ear artery)
Heart
Intravenous,
Intra-arterial
Tail vein
Carotid artery
Cylinder
External (in the chest)
Internal (on the chest)
Liver
Intravenous,
Intra-arterial
Tail vein
Carotid artery
Cylinder,
Square
External (on the right flank)
Internal (for focalized gene transfer)
Lung
Intravenous
Tail vein
Square
External
Intestine
Ileum lumen
Intestine
Cylinder,
Square
Internal
Brain
Intraventricular
Brain ventricle
Small Cylinder
External

The only requirement for In vivo Magnetofection™ is a small magnet specifically designed for this application. Several kinds of magnets are provided depending of your application.

Products

Cat. No.
DescriptionAmount

In vivo PolyMag

IV-TK30210
In Vivo PolyMag Transfection Trial Kit
1 kit
IV-KC30210
In Vivo PolyMag Transfection Starting Kit
1 kit
IV-PN30500
In Vivo PolyMag 500 Transfection Reagent
500 ul
IV-PN31000
In Vivo PolyMag 1000 Transfection Reagent
1 ml

In vivo DogtorMag

IV-TK30220
In Vivo DogtorMag Transfection Trial Kit
1 kit
IV-KC30220
In Vivo DogtorMag Transfection Starting Kit
1 kit
IV-DM30500
In Vivo DogtorMag 500 Transfection Reagent
500 ul
IV-DM31000
In Vivo DogtorMag 1000 Transfection Reagent
1 ml

In vivo ViroMag

IV-TK30230
In Vivo ViroMag Transfection Trial Kit
1 kit
IV-KC30230
In Vivo ViroMag Transfection Starting Kit
1 kit
IV-VM30250
In Vivo ViroMag 250 Transfection Reagent
250 ul
IV-VM30500
In Vivo ViroMag 500 Transfection Reagent
500 ul

In vivo SilenceMag

IV-TK30240
In Vivo SilenceMag Trial Kit
1 kit
IV-KC30240
In Vivo SilenceMag 500 Starting Kit
1 kit
IV-SM30500
In Vivo SilenceMag 500 Transfection Reagent
500 ul
IV-SM31000
In Vivo SilenceMag 1000 Transfection Reagent
1 ml



Source:
Website: OZ Biosciences

Related to:
Brands: OZ Biosciences
News: Magnetofection™
Product groups: Molecular Biology