Influenza A H5N3 Hemagglutinin (HA1 Subunit) Human Cells Transfected Lysate | 11696-V08H1L-300

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Product specifications for - Influenza A H5N3 (A/duck/Hokkaido/167/2007) Hemagglutinin (HA1 Subunit) Human Cells Transfected Lysate (positive control) (denatured)

Overview:
Product group:	Lysates / Extracts
Category:	Lysates
Application:	Western Blot
Application note:	WB: Use at an assay dependent dilution.Not yet tested in other applications.Optimal dilutions/concentrations should be determined by the end user.
Species:	Virus
Target:	Spta1
Target description:	spectrin alpha, erythrocytic 1

Properties:
Datasheet:	Datasheet
	Research Use Only
UNSPSC:	41105330
Storage instructions:	Store at -80-¦C. Aliquot to avoid repeated freezing and thawing

Scientific information:
Scientific info:	Human Cells transfected lysate in which Influenza A H5N3 (A/duck/Hokkaido/167/2007) Hemagglutinin (HA1 Subunit) has been over-expressed. The whole cell lysate is provided in 1X Sample Buffer. Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized; Stability: Samples are stable for up to twelve months from date of receipt at -80°C
Gene ID:	20739

Scientific information:

Scientific info:

Human Cells transfected lysate in which Influenza A H5N3 (A/duck/Hokkaido/167/2007) Hemagglutinin (HA1 Subunit) has been over-expressed. The whole cell lysate is provided in 1X Sample Buffer. Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized; Stability: Samples are stable for up to twelve months from date of receipt at -80°C

Gene ID:

20739

Additional information:
Synonyms:	11696-V08H1L-300; AF093576; AI451697; Spna-1; Spna1; ha; ihj; nmf4; sph; Spta1; spectrin alpha, erythrocytic 1; alpha-spectrin 1, erythroid; erythroid alpha-spectrin; hemolytic anemia; neuroscience mutagenesis facility, 4; spectrin alpha 1; spectrin alpha chain, erythrocyte; spectrin alpha chain, erythrocytic 1; spherocytosis

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Influenza A H5N3 (A/duck/Hokkaido/167/2007) Hemagglutinin (HA1 Subunit) Human Cells Transfected Lysate (positive control) (denatured)

Product specifications for - Influenza A H5N3 (A/duck/Hokkaido/167/2007) Hemagglutinin (HA1 Subunit) Human Cells Transfected Lysate (positive control) (denatured)