Magnetofection™

Magnetofection™ method - OZ BiosciencesMagnetofection™ is a novel, simple and highly efficient transfection method. Inspired by the validated and recognized magnetic drug targeting technology, this original method is a revolution for transfection and infection. In essence, the idea was to unite the advantages of the popular biochemical (cationic lipids or polymers) and physical (electroporation, gene gun) transfection methods in one system while excluding their inconveniences (low efficiency, toxicity, difficulty to handle). It is the unique technology suitable for viral and non viral gene delivery applications.

Principle

Magnetofection™ principle is to associate nucleic acids, transfection reagents or viruses with specific magnetic nanoparticles. The resulting molecular complexes are then concentrated and transported into cells supported by an appropriate magnetic field. In this way, the exploitation of a magnetic force exerted upon gene vectors allows a very rapid concentration of the entire applied vector dose on cells, so that 100% of the cells get in contact with a significant vector dose, and promotes cellular uptake.

How does it work?

The magnetic nanoparticles are made of iron oxyde, which is fully biodegradable, coated with specific proprietary cationic molecules varying upon applications. Their association with the gene vectors (DNA, siRNA, ODN, virus, etc.) is achieved by salt-induced colloidal aggregation and electrostatic interaction. The magnetic particles are then concentrated onto cells by the influence of an external magnetic field generated by a specific magnetic plate. The cellular uptake of the genetic material is accomplished by endocytosis and pinocytosis, two natural biological processes. Consequently, membrane architecture & structure stay intact in contrast to other physical transfection methods that damage, create hole or electroshock the cell membranes. The nucleic acids are then released into the cytoplasm by different mechanisms depending upon the formulation used.
  1. First is the proton sponge effect caused by cationic polymers coated on the nanoparticles that promotes endosome osmotic swelling, disruption of the endosomal membrane and intracellular release of DNA.
  2. Second is the destabilization of the endosome by cationic lipids coated on the particles that release the nucleic acid into cells by flip-flop of cell negative lipids and charged neutralization.
  3. Third one is the usual viral mechanism when virus is used.


Biodistribution of Magnetic Nanoparticles

The biodegradable cationic magnetic nanoparticles are not toxic at the recommended doses and even higher. Gene vectors / magnetic nanoparticles complexes are internalized into cells after 10-15 minutes i.e. much faster than any other transfection method. After 24, 48 or 72 hours, most of the particles are localized in the cytoplasm, in vacuoles (membranes surrounded structure into cells) and occasionally in the nucleus. In addition, magnetic nanoparticles do not influence cell function.

What are the applications?

Magnetofection™ is the only versatile and universal technology adapted to all types of nucleic acids (DNA, siRNA, dsRNA, shRNA, mRNA, ODN...), non viral transfection systems (transfection reagents) and viruses. Consequently, several optimized reagents have been designed according to defined applications:
  • LipoMag Kit: for all nucleic acids, association of CombiMag + DreamFect Gold reagents
  • MagnetoFectamine O2: for primary and hard-to-transfect cells
  • PolyMag / PolyMag Neo: for all nucleic acids transfection
  • NeuroMag: for neurons transfection
  • CombiMag: for enhancing all transfection reagents efficiency (see Magnetofectamine)
  • SilenceMag: for siRNA applications
  • ViroMag: for enhancing viral transduction efficiency
  • ViroMag R/L: for Lentivirus and Retrovirus transduction
  • AdenoMag: for Adenovirus and AAV transduction
  • Mag4C-LV / Mag4C-AD: for capturing and concentrating Lentiviruses and Adenoviruses
  • FluoMag: Fluorescent Magnetofection Reagents
  • SelfMag: for creating your own magnetic delivery system
Magnetofection™ has been successfully tested on a broad range of cell lines, hard-to-transfect and primary cells. It is perfect for non-dividing or slowly dividing cells, meaning that the genetic materials can go to the nucleus without cell division. We have shown that combining magnetic nanoparticles to gene vectors of any kind results in a dramatic increase of uptake of these vectors and high transfection efficiency. It is the only technology suitable both for viruses and non-viral nucleic acid delivery applications.
- For non viral nucleic acid delivery, it is perfect for primary and hard-to-transfect adherent cells.
- For viral applications, it is ideal for any cells including primary cells (adherent and suspension).

Please contact our technical support: techsupport(at)bio-connect.nl for the list of cells successfully tested.

Magnetofection™ reagent selection guide


 Product
DNAmRNAsiRNA/
miRNA
Applications
in vitro Magnetofection™
Primary and Hard-to-transfect Cells

CombiMag

Enhance transfection efficiency of any transfection reagents
LipoMag

  A combination of DreamFect Gold™ and CombiMag reagents
MagnetoFectamine

  A combination of LipoFectamine 2000™ and CombiMag reagents
PolyMag Neo



Polymer-based Magnetofection reagent
NeuroMag



Specifically designed for primary neurons and neural cells
SilenceMag
  
Highly efficient for siRNA transfection
in vivo Magnetofection™
in vivo DogtorMag


 in vivo lipid-based transfection reagent
in vivo PolyMag

  in vivo polymer-based transfection reagent
in vivo SilenceMag
  
in vivo gene silencing applications

How do I use Magnetofection™ reagents?

The protocol is a very straightforward and easy procedure:
  1. Dilute nucleic acids or vectors in serum free medium or buffer and add Magnetofection™ reagent.
  2. Incubate 20-30 minutes.
  3. Add these complexes directly to cells.
  4. Apply the magnetic field (place the culture plate on the magnetic plate).
  5. Incubate 5-20 minutes, remove the magnetic plate and culture cells until assay.

Do I need specific equipments?

The only requirement for Magnetofection™ is a magnetic plate specifically designed for this application.The magnetic plate is a one-time buy and completely reusable, so you do not need expensive equipment contrary to approaches such as electroporation or gene gun. Basically, the magnetic field required is produced by specific magnets. Three magnetic plates are available: Super Magnetic Plate, Magnetic Plate with 96 individuals magnets and Mega Magnetic Plate. Their design allows producing a heterogeneous magnetic field that magnetizes the nanoparticles in solution, forms a very strong gradient to attract the nanoparticles and covers all the surface of the plate. The plate can be washed with ethanol 70% and used within incubators or robots.

Products

Cat. No.DescriptionAmount

LipoMag

LM80250LipoMag Kit 250 Magnetic Transfection Reagent3 kits
LM80500LipoMag Kit 500 Magnetic Transfection Reagent4 kits
LM80000LipoMag Kit Transfection Starter Kit2 kits

Magnetofectamine O2

MTX2-0750MagnetoFectamine O2 Transfection Kit
1 kit
MTX2-1000MagnetoFectamine O2 Starting Kit1 kit

PolyMag

PN30100PolyMag 100 Magnetic Transfection Reagent100 ul
PN31000PolyMag 1000 Magnetic Transfection Reagent1 ml
PN30200PolyMag 200 Magnetic Transfection Reagent200 ul
KC30200Starting Kit with Super Magnetic Plate + 100µL of CombiMag, PolyMag, PolyMag Neo1 kit

PolyMag Neo

PG60100PolyMag Neo 100 Magnetic Transfection Reagent100 ul
PG61000PolyMag Neo 1000 Magnetic Transfection Reagent1 ml
PG60200PolyMag Neo 200 Magnetic Transfection Reagent200 ul
KC30200Starting Kit with Super Magnetic Plate + 100µL of CombiMag, PolyMag, PolyMag Neo1 kit

NeuroMag

NM51000NeuroMag 1000 Magnetic Transfection Reagent1 ml
NM50200NeuroMag 200 Magnetic Transfection Reagent200 ul
NM50500NeuroMag 500 Magnetic Transfection Reagent500 ul
KC30800Starting Kit with Super Magnetic plate + 200µL of NeuroMag1 kit

CombiMag

CM20100CombiMag 100 Transfection Reagent100 ul
CM21000CombiMag 1000 Transfection Reagent1 ml
CM20200CombiMag 200 Transfection Reagent200 ul
KC30200Starting Kit with Super Magnetic Plate + 100µL of CombiMag, PolyMag, PolyMag Neo1 kit

SilenceMag

SM11000SilenceMag 1000 Transfection Reagent1 ml
SM10200SilenceMag 200 Transfection Reagent200 ul
SM13000SilenceMag 3000 Transfection Reagent3 x 1 ml
SM10500SilenceMag 500 Transfection Reagent500 ul
KC30300siRNA Starting Kit with Super Magnetic plate + 200µL of SilenceMag1 kit
KC30400Super Starting Kit with Super Magnetic plate + 100µL of PolyMag, CombiMag, PolyMag Neo & 200µL of SilenceMag1 kit

ViroMag

VM40100ViroMag 100 Magnetic Transduction Reagent100 ul
VM41000ViroMag 1000 Magnetic Transduction Reagent1 ml
VM40200ViroMag 200 Magnetic Transduction Reagent200 ul
KC30500
ViroMag Starting Kit with Super Magnetic plate + 200µL of ViroMag
1 kit
KC30600
ViroMag Triple Starting Kit with Super Magnetic plate + 100µL of ViroMag, AdenoMag, ViroMag R/L
1 kit

ViroMag R/L

RL40100ViroMag R/L 100 Magnetic Transduction Reagent100 ul
RL41000ViroMag R/L 1000 Magnetic Transduction Reagent1 ml
RL40200ViroMag R/L 200 Magnetic Transduction Reagent200 ul
KC30700
ViroMag R/L Starting Kit with Super Magnetic plate + 200µL of ViroMag R/L
1 kit

AdenoMag

AM70100AdenoMag 100 Magnetic Transduction Reagent100 ul
AM71000AdenoMag 1000 Magnetic Transduction Reagent1 ml
AM70200AdenoMag 200 Magnetic Transduction Reagent200 ul
KC30900AdenoMag Starting Kit with Super Magnetic Plate + 200µL of AdenoMag1 kit

Mag4C-LV

LKC11000Mag4C-LV Kit1 kit
LTK11200Mag4C-LV Trial Kit1 kit

Mag4C-AD

AKC11000Mag4C-AD Kit1 kit
ATK11200Mag4C-AD Trial Kit1 kit

FluoMag

FC10100FluoMag-C 100 Transfection Reagent100 ul
FN10100FluoMag-N 100 Transfection Reagent100 ul
FP10100FluoMag-P 100 Transfection Reagent100 ul
FS10100FluoMag-S 100 Transfection Reagent100 ul
FV10100FluoMag-V 100 Transfection Reagent100 ul

SelfMag

SF40000Magfectin Transfection Reagent1 ml
DM30000MAG-ID Magnetic Rack1 rack
SA11000SelfMag Amino Beads Transfection1 ml
SA10000SelfMag Amino Transfection Kit1 kit
SC21000SelfMag Carboxy Transfection Beads1 ml
SC20000SelfMag Carboxy Transfection Kit1 kit
SA12000Transfection Buffer Kit A1 kit
SC22000Transfection Buffer Kit C1 kit



Source:
Brochure: Magnetofection™
Website: OZ Biosciences

Related to:
Brands: OZ Biosciences
News: In vivo Magnetofection™​
Product groups: Chemicals, Labware / AccessoriesMolecular Biology