Mebiol® Gel PNIPAAm-PEG 3D Thermoreversible Hydrogel

Hydrogels are a diverse class of polymeric materials characterized by their network-like structure and high water content. Hydrogels of many kinds have found a wide variety of applications in medicine and life science research weighted towards, but not at all limited to three-dimensional cell culture, tissue engineering, and drug delivery. Properties highly favorable to cell culture and tissue engineering applications prompted the commercialization of Mebiol® Gel, a copolymer of poly(N-isopropylacrylamide) and poly(ethylene glycol) (PNIPAAm-PEG) for research purposes in the early 2000's.


Mebiol® Gel's defining feature, in contrast to other commercially available hydrogels, is its temperature reversible sol-gel transition. When cooled, Mebiol® Gel is a sol (handles like a liquid) but becomes a rigid hydrogel at higher temperatures. In practice, this means extremely easy cell handling. Cultures are seeded into cooled Mebiol® Gel and recovered conveniently by cooling the culture vessel and centrifugation. In the gel state, the highly lipophylic environment of the Mebiol® Gel presents an efficient niche for cell proliferation, cell communication, gas and mass exchange, and protection of cells and tissue from shear forces.

Mebiol® Gel Features

  • Easy handling
  • Non-toxic, biocompatible
  • 100% synthetic, pathogen free
  • High transparency for cell observation
  • Proven performance

Mebiol® Gel published applications include:

  • Stem cell and pluripotent stem cell culture, expansion, and differentiation
  • Spheroid culture
  • Cell implantation
  • Organ and tissue regeneration
  • Drug delivery
  • Non-cell culture application
  • Physical properties

Application examples

1. Culture of primary cancer cells in Mebiol® Gel

In collagen and other 3D gel cultures, proliferation of primary cancer cells may be inhibited by fibroblast overgrowth. Importantly, fibroblasts do not readily grow in Mebiol® Gel, allowing selective proliferation of primary cancer cells for further analysis and characterization.

Figure 1: Cancerous human colon tissue cultured in Mebiol® Gel for 10 days. (Courtesy of Dr. S. Kubota, Dept. of General Surgery, St. Marianna University School of Medicine)

 

2. Stem Cell Culture

Macaca embryonic stem cells cultured on Mebiol® Gel without LIF (right panel) show morphology and alkaline phosphatase staining characteristic of undifferentiation compared to 2D feeder layer cultures (left panel).

Figure 2: Courtesy of Dr. K. Hishikawa, Dept of Clinical Renal Regeneration, University of Tokyo.

3. Spheroid Formation

Mebiol® Gel supports spheroid formation of cancer cell lines and iPS cells.

Figure 3: Spheroid formation in Mebiol® Gel by a cell line derived from mucoepidermoid carcinoma (cholangioma). (Courtesy of Dr. S. Kubota, Dept. of General Surgery, St. Marianna University School of Medicine)
 

4. Tissue Structure Preservation

Mebiol® Gel's protective environment helps to preserve tissue structure over long term culture.

Figure 4: Left panel: Normal colonic mucosal tissue after culture on Mebiol® Gel for 7 days. Right panel: Metastatic hepatic carcinoma tissue after culture in Mebiol® Gel for 21 days.

(Courtesy of Dr. S. Kubota, Dept. of General Surgery, St. Marianna University School of Medicine)

Preparation

Mebiol® Gel Usage (refer to package insert for details)

① Rehydration

Add 10 ml/50 ml of cold aqueous solution suitable to your application (e.g. culture medium, PBS). Insure all powder is wetted. Lay flask flat and stationalry for several hours at 4℃, with occasional gentle shaking.


 

② Cell Culture and Recovery

Add cells or tissue to Mebiol Gel in the sol state (cold). Culture at 37℃. Recover cells or tissue by cooling culture vessel and diluting with 30-40 ml/150-200 ml cold fluid to prevent gelation. Centrifuge in cold.



Dynamic Viscoelastic Property of Mebiol® Gel


Temperature dependence of the dynamic moduli of the aqueous solution of Mebiol® Gel at a concentration of 10 wt% in distilled water. Storage modulus (G', solid lines) and loss modulus (G'', broken lines) were measured on heating (closed symbols) and cooling (open symbols) at the oscillatory frequency of 1 Hz.

Products

Cat. No.
DescriptionAmount
MBG-PMW20-1001
Mebiol® Gel
1 x 10 ml
MBG-PMW20-1005
5 x 10 ml
MBG-PMW20-5001
1 x 50 ml
MBG-PMW20-5005
5 x 50 ml



Source:
Website: Cosmo Bio

Related to:
Brands: Cosmo Bio
Product groups: Technique Specific Products