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Aminoallyl-dUTP-XX-Cy3

Research Use Only
NU-803-XX-CY3-L
Jena Bioscience
Product group Molecular Biology
€ 493,00
5 x 10 ul
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Overview

  • Supplier
    Jena Bioscience
  • Product Name
    Aminoallyl-dUTP-XX-Cy3
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Scientific Description
    Aminoallyl-dUTP-XX-Cy3 is recommended for direct enzymatic labeling of DNA/cDNA e.g. by PCR and Nick Translation. It is incorporated as substitute for its natural counterpart dTTP. The resulting Dye-labeled DNA/cDNA probes are ideally suited for fluorescence hybridization applications such as FISH or microarray-based gene expression profiling. Optimal substrate properties and thus labeling efficiency is ensured by an optimized linker attached to the C5 position of uridine. Higher incorporation and thus labeling rates can be achieved compared to Aminoally-dUTP-Cy3 (NU-803-Cy3). Recommended Aminoallyl-dUTP-XX-Cy3/dTTP ratio for PCR and Nick Translation: 30-50% Aminoallyl-dUTP-XX-Cy3/ 50% dTTP Please note: Protect the Dye-labeled dUTP from exposure to light and carry out experimental procedures in low light conditions. The optimal final concentration of the Dye-labeled dUTP may very depending on the application and assay conditions. For optimal produdct yields and high incorporation rates an individual optimization of the Dye-labeled-dUTP/dTTP ratio is recommended.
  • Storage Instruction
    -20°C
  • UNSPSC
    41106305