Nuclease P1 (from Penicillium citrinum)

JaICA

Nuclease P1 (from Penicillium citrinum)

10

Research Use Only

Catalyzes the nonspecific endonucleolytic cleavage of single stranded DNA and RNA to yield nucleoside 5-phosphates and 5-phospho-oligonucleotides. Nuclease P1 hydrolyzes both 3-5-phosphodiester bonds in RNA and heat-denatured DNA and 3-phosphomonoester bonds in mono- and oligonucleotides terminated by 3-phosphate without base specificity. The enzyme does not attack double-stranded nucleic acids, especially in the presence of more than 400mmol/l of sodium chloride at pH 6.0. - Catalyzes the nonspecific endonucleolytic cleavage of single stranded DNA and RNA to yield nucleoside 5-phosphates and 5-phospho-oligonucleotides. The enzyme has an optimal temperature of approximately 70 °C, but for a long incubation, a temperature below 60 °C is more suitable. It is stable in the pH range of 5 - 8.

2°C to 8°C

41116133