The enzyme-linked immunospot (ELISPOT) assay, first described in 1983, is a powerful evolution of the classic ELISA immunoassay. While ELISA measures bulk cytokine or antibody levels in a sample, ELISPOT detects and quantifies individual cytokine‑secreting T cells or antibody‑secreting B cells at the single‑cell level. Because plate‑bound antibodies capture proteins immediately as they are secreted, … Continue reading ELISPOT Assay: From ELISA To Single-Cell Immune Monitoring