DNA/RNA Damage Assays

Easily measure distinct types of nucleic acid damage in various assay formats

DNA is arguably one of the most biologically significant targets of cellular stress. DNA may be damaged by a variety of endogenous and exogenous sources, and the damage may take various forms:
  • Oxidative DNA damage refers to the oxidation of specific bases
  • Hydrolytic DNA damage involves the deamination or total removal of individual bases
  • DNA strand breaks involve a cut in one or both strands; double-strand breaks are especially dangerous and can be mutagenic
  • Pyrimidine dimers are formed most often from ultraviolet radiation
  • Polycyclic aromatic hydrocarbons are often formed from exposure to chemical carcinogens

Universal DNA Damage: Comet Assay

The Comet Assay is a well-published method to detect various types of DNA damage in intact cells by electrophoresis. Damaged DNA is easily distinguished from healthy DNA by light microscopy; the damaged DNA moves farther when current is applied, creating a “comet” shape (see below).


Jurkat Cells Treated without (left) and with (right) Etoposide using the OxiSelect™ Comet Assay Kit and Analyzed by Light Microscopy.

Cat. No.
DescriptionAmount
STA-350
OxiSelect Comet Assay Kit (3-Well Slides)
15 assays
STA-351
OxiSelect Comet Assay Kit (3-Well Slides)
75 assays
STA-351-5
OxiSelect Comet Assay Kit (3-Well Slides)
5 x 75 assays
STA-352
OxiSelect Comet Assay Slides (3-Well)
5 slides
STA-353
OxiSelect Comet Assay Slides (3-Well)
25 slides
STA-353-5
OxiSelect Comet Assay Slides (3-Well)
5 x 25 slides
STA-355
OxiSelect 96-Well Comet Assay Kit
96 assays
STA-355-5
OxiSelect 96-Well Comet Assay Kit
5 x 96 assays
STA-356
OxiSelect 96-Well Comet Assay Slide
1 slide
STA-356-5
OxiSelect 96-Well Comet Assay Slide
5 slides
STA-354
OxiSelect Comet Assay Control Cells
1 set

Oxidative DNA Damage: 8-OHdG

8-hydroxydeoxyguanosine (8-OHdG) is the most common marker for oxidative DNA damage and can be measured in virtually any species. It is formed and enhanced most often by chemical carcinogens.

The OxiSelect™ Oxidative DNA Damage ELISA provides a highly sensitive method for measuring 8OHdG formation. Detect as little as 100 pg/mL in DNA isolated from urine, serum, cells and tissues.

Cat. No.
DescriptionAmount
STA-320
OxiSelect Oxidative DNA Damage ELISA Kit (8-OHdG Quantitation)
96 assays
STA-320-5
OxiSelect Oxidative DNA Damage ELISA Kit (8-OHdG Quantitation)
5 x 96 assays
STA-320-T
OxiSelect Oxidative DNA Damage ELISA Kit (8-OHdG Quantitation), Trial Size
32 assays

Oxidative RNA Damage: 8-OHG

Like its DNA counterpart 8-OHdG above, 8-OHG (8-hydroxyguanosine) is the most common marker for oxidative RNA damage.

The OxiSelect™ Oxidative RNA Damage ELISA has a detection limit of 300 pg/mL and is suitable for use with cells, tissues, serum, urine or cerebrospinal fluid.

Cat. No.
DescriptionAmount
STA-325
OxiSelect Oxidative RNA Damage ELISA Kit (8-OHG Quantitation)
96 assays
STA-325-5
OxiSelect Oxidative RNA Damage ELISA Kit (8-OHG Quantitation)
5 x 96 assays
STA-325-T
OxiSelect Oxidative RNA Damage ELISA Kit (8-OHG Quantitation), Trial Size
32 assays

Hydrolytic DNA Damage: AP Sites

Loss of DNA bases can be particularly mutagenic, and if left unrepaired these AP (apurinic /apyrimidinic) sites can inhibit transcription. The OxiSelect™ DNA Damage Quantitation Kit (AP sites) provides a highly sensitive method for quantitation of AP sites. Quantify as few as 4-40 AP sites per 105 bp of DNA in tissue or cell lysates.

Cat. No.
DescriptionAmount
STA-324
OxiSelect Oxidative DNA Damage Quantitation Kit (AP sites)
50 assays
STA-324-T
OxiSelect Oxidative DNA Damage Quantitation Kit (AP Sites), Trial Size
10 assays

DNA Double-Strand Breaks

Double-strand breaks (DSB) in DNA are among the most dangerous types of DNA damage. One of the first responses to DSBs in mammalian cells is the phosphorylation of the Ser139 residue of a histone variant, H2AX, which occurs within seconds at the site of the damage. This phosphorylation causes chromatin condensation and appears to play an important role in recruitment of repair factors.

The OxiSelect™ DNA Double-Strand Break Staining Kit detects DSBs in cultured cells.


DNA Double-Strand Break Detection in Untreated (left) and Etoposide-Treated (right) A549 Cells using the OxiSelect™ DNA Double-Strand Break Staining Kit.

Cat. No.
DescriptionAmount
STA-321
OxiSelect DNA Double-Strand Break (DSB) Staining Kit
100 assays
STA-321-T
OxiSelect DNA Double Strand Break (DSB) Staining Kit, Trial Size
20 assays

UV-Induced Damage:  Pyrimidine Dimers

Absorption of ultraviolet radiation can damage DNA by the formation of pyrimidine dimers. The two main forms of pyrimine dimers are cyclobutane pyrimidine dimers (CPD) and pyrimidine (6-4) pyrimidone photoproducts (6-4PP).
  • ELISA kits to measure CPD or 6-4PP in DNA samples isolated from cells or tissues
  • Cell-Based ELISA kits for use on intact cells
  • Immunostaining kits for visualization by fluorescence microscopy
Cat. No.
DescriptionAmount
STA-323
OxiSelect UV-Induced DNA Damage ELISA Kit (6-4PP Quantitation)
96 assays
STA-323-5
OxiSelect UV-Induced DNA Damage ELISA Kit (6-4PP Quantitation)
5 x 96 assays
STA-323-T
OxiSelect UV-Induced DNA Damage ELISA Kit (6-4PP Quantitation), Trial Size32 assays
STA-322
OxiSelect UV-Induced DNA Damage ELISA Kit (CPD Quantitation)
96 assays
STA-322-5
OxiSelect UV-Induced DNA Damage ELISA Kit (CPD Quantitation)5 x 96 assays
STA-322-T
OxiSelect UV-Induced DNA Damage ELISA Kit (CPD Quantitation), Trial Size
32 assays
STA-322-C
OxiSelect UV-Induced DNA Damage ELISA Combo Kit (CPD / 6-4PP Quantitation)
96 assays
STA-328
OxiSelect Cellular UV-Induced DNA Damage ELISA Kit (6-4PP)
96 assays
STA-328-T
OxiSelect Cellular UV-Induced DNA Damage ELISA Kit (6-4PP), Trial Size
32 assays
STA-326
OxiSelect Cellular UV-Induced DNA Damage ELISA Kit (CPD)
96 assays
STA-326-5
OxiSelect Cellular UV-Induced DNA Damage ELISA Kit (CPD)
5 x 96 assays
STA-326-T
OxiSelect Cellular UV-Induced DNA Damage ELISA Kit (CPD), Trial Size
32 assays
STA-329
OxiSelect Cellular UV-Induced DNA Damage Staining Kit (6-4PP)
96 assays
STA-329-T
OxiSelect Cellular UV-Induced DNA Damage Staining Kit (6-4PP), Trial Size
32 assays
STA-327
OxiSelect Cellular UV-Induced DNA Damage Staining Kit (CPD)
96 assays
STA-327-T
OxiSelect Cellular UV-Induced DNA Damage Staining Kit (CPD), Trial Size
32 assays

BPDE DNA Adduct Assay

Benzo(a)pyrene diol epoxide (BPDE) is a mutagen that may create adducts with DNA as well as proteins. It is a derived from benzo(a)pyrene which is polycyclic aromatic hydrocarbon (PAH), a family of chemical carcinogens commonly found in environmental pollution.


Chemical Conversion of Benzo(a)pyrene to Benzo(a)pyrene 7,8-diol-9,10 epoxide (BPDE).

Cat. No.
Description
Amount
STA-357
OxiSelect BPDE DNA Adduct ELISA Kit
96 assays

For more information about all protein oxidation & nitration assays to accurately measure oxidative and nitrative protein damage, Lipid peroxidation assays to quantify stable by-products of lipid peroxidation, reactive oxygen species (ROS) assays to measure ROS with high sensitivity in intact cells, cell lystaes or blood samples and all antioxidant assays for quantifying antioxidant capacity or specific antioxidant enzymes please contact our technical support.



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Brands: Cell Biolabs
Product groups: Assays