TGFbeta1 (mutant) (human):Fc (human) (rec.)

Chimerigen Laboratories

TGFbeta1 (mutant) (human):Fc (human) (rec.)

10

Research Use Only

>98%

Protein. The extracellular domain of a mutant human TGFbeta1 is fused to the N-terminus of the Fc region of human IgG4. Site-directed mutagenesis was used to change three cysteine codons into a serine codon that are located in the pro region of the TGF-beta precursor at amino acid positions 33, 223, and 225. Source: CHO cells. Endotoxin content: <0.06EU/microg protein (LAL test; Lonza). Lyophilized from 0.2microm-filtered solution in PBS. Binds human TGFbeta. Shown to cross-react with mouse TGFbeta (see lit. reference 1). Purity: >98% (SDS-PAGE). TGF-beta1 (Transforming growth factor beta 1) is a polypeptide member of the transforming growth factor beta superfamily of cytokines. It is a secreted protein that performs many cellular functions, including the control of cell growth, cell proliferation, cell differentiation and apoptosis. In humans, TGF-beta1 is encoded by the TGFB1 gene. TGF-beta1 was first identified in human platelets as a protein with a molecular mass of 25 kaD with a potential role in wound healing. It was later characterized as a large protein precursor (containing 390 aa) that was proteolytically processed to produce a mature peptide of 112 aa. TGF-beta1 plays an important role in controlling the immune system, and shows different activities on different types of cell, or cells at different developmental stages. Most immune cells (or leukocytes) secrete TGF-beta1. - TGF-beta1 (Transforming growth factor beta 1) is a polypeptide member of the transforming growth factor beta superfamily of cytokines. It is a secreted protein that performs many cellular functions, including the control of cell growth, cell proliferation, cell differentiation and apoptosis. In humans, TGF-beta1 is encoded by the TGFB1 gene. TGF-beta1 was first identified in human platelets as a protein with a molecular mass of 25 kaD with a potential role in wound healing. It was later characterized as a large protein precursor (containing 390 aa) that was proteolytically processed to produce a mature peptide of 112 aa. TGF-beta1 plays an important role in controlling the immune system, and shows different activities on different types of cell, or cells at different developmental stages. Most immune cells (or leukocytes) secrete TGF-beta1. Normally, TGF-beta1 is secreted as a complex with Latency-Associated Peptide (LAP) that is inactive. Removal of LAP activates TGF-beta1 that is cleared quickly from the circulation. This specific mutant protein containing the LAP+TGF-beta1 domains including the indicated three mutated cysteines, allows the protein to be active and to have long lasting biological activity with increased stability and half-life time.

2°C to 8°C,-20°C

12352202