More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/TRF-Ubiquitin Mix (100X) (2000 reactions)
% Signal to Background of Continuous Real-Time TR-FRET E3 Ligase titration (autoubiquitination): An example using serial dilutions of GST-MDM2 from 200nM to 12.5nM mixed with UBA1, UBE2D3 and trf-Ub mix. Reaction was initiated with addition of Mg-A
% Signal to Background of Continuous Real-Time TR-FRET E3 Ligase titration (autoubiquitination): An example using serial dilutions of GST-MDM2 from 200nM to 12.5nM mixed with UBA1, UBE2D3 and trf-Ub mix. Reaction was initiated with addition of Mg-A
% Signal to Background of Continuous Real-Time TR-FRET E3 Ligase titration (autoubiquitination): An example using serial dilutions of GST-MDM2 from 200nM to 12.5nM mixed with UBA1, UBE2D3 and trf-Ub mix. Reaction was initiated with addition of Mg-A

TRF-Ubiquitin Mix (100X) (2000 reactions)

Research Use Only
SBB-TR0051-2K
South Bay Bio
DownloadDatasheet
Product group Proteins / Signaling Molecules
Price on request
Packing Size
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    South Bay Bio
  • Product Name
    TRF-Ubiquitin Mix (100X) (2000 reactions)
  • Delivery Days Customer
    10
  • Certification
    Research Use Only
  • Estimated Purity
    >99%
  • Scientific Description
    Protein. Souce: E. coli. Liquid. In 50mM HEPES pH 7.5, 100mM sodium chloride. Ubiquitin is a highly conserved protein that plays a major role in the ubiquitination pathway, which is conserved from yeast to mammals. Ubiquitination, the conjugation of ubiquitin to other proteins through a covalent bond between its C-terminal glycine and the epsilon-amino group of lysine residues or the alpha-amino group of an N- terminal methionine onto proteins is essential for many cellular processes primarily linked to protein degradation. This process involves three steps with specific groups of enzymes in an ATP depended manner, which are activation with ubiquitin-activating enzymes (E1s), conjugation with ubiquitin-conjugating enzymes (E2s) and ligation with ubiquitin ligases (E3s). This product is a mix of Europium-Cryptate Ubiquitin (SBB-TR0014), Cy5-Ubiquitin (SBB-TR0015), and wild-type Ubiquitin (SBB-UP0013) in a ratio optimized for TR-FRET based conjugation experiments where long polyubiquitin chains are formed. Under conditions where short ubiquitin chains are formed, a different mix may be required to optimize signal to background ratio. Enzymatic incorporation of the labeled ubiquitins into chains conjugated onto a substrate protein leads to an increase in fluorescence emission at 665nm (Em-Acceptor) and decrease at emission wavelength 620nm (Em-Donor). - Ubiquitin is a highly conserved protein that plays a major role in the ubiquitination pathway, which is conserved from yeast to mammals. Ubiquitination, the conjugation of ubiquitin to other proteins through a covalent bond between its C-terminal glycine and the epsilon-amino group of lysine residues or the alpha-amino group of an N- terminal methionine onto proteins is essential for many cellular processes primarily linked to protein degradation. This process involves three steps with specific groups of enzymes in an ATP depended manner, which are activation with ubiquitin-activating enzymes (E1s), conjugation with ubiquitin-conjugating enzymes (E2s) and ligation with ubiquitin ligases (E3s).
  • Storage Instruction
    -20°C,-80°C
  • UNSPSC
    12352202