VISTA (mouse):COMP (mouse) (rec.) (His)

Catalog number: AG-40B-0181-C050
Brand: AdipoGen Life Sciences
Packing: 50 ug
Price: € 400.00
Expected delivery time: 7 days
Quantity:

Product specifications for - VISTA (mouse):COMP (mouse) (rec.) (His)

Overview: 
Product group: Proteins / Signaling Molecule
Category: Proteins
Species: Mouse
Target: Vsir
Target description: V-set immunoregulatory receptor
Source: 293 Cells
Properties: 
Purity: >95% (SDS-PAGE)
Molecular weight: ~35-40kDa (SDS-PAGE) and >250kDa (Size Exclusion Chromatography (SEC))
Datasheet: Datasheet
  Research Use Only
UNSPSC: 12352202
Concentration: 1 mg/ml
Storage instructions: -20-¦C
Scientific information: 
Scientific info: Mouse VISTA is a 55 to 65kDa type I Ig membrane protein with the extracellular domain homologous to PD-L1. VISTA is mainly expressed on hematopoietic tissues (spleen, thymus and bone marrow) and on myeloid cells with lower expression on CD4+ and CD8+ T cells. VISTA is a new negative checkpoint regulator that potently suppresses T cell activation. Overexpression of VISTA on tumors inhibits the protective anti-tumor immunity and blockade of VISTA enhances antitumor immunity in multiple tumor models. VISTA on APC delivers negative signaling to T cells via a yet-to-identify binding partner on T cells. Recently, it has been reported that recombinant VISTA protein needs to be multimerized to be active as soluble ligand. The protein VISTA (mouse):COMP (mouse) (with the extracellular domain of VISTA (mouse) fused to the pentamerization domain from the cartilage oligomeric matrix protein (COMP)), but not VISTA-Fc, functions as an immunosuppressive agonist in vivo inhibiting the proliferation of CD4+ T cells. - Protein. The extracellular domain of mouse VISTA (aa 33-191) is fused at the C-terminus to the domain of the mouse cartilage oligomeric matrix protein (COMP) (aa 28-72) and a His Tag. Source: HEK 293 cells. Endotoxin content: <0.01EU/microg purified protein (LAL test; Lonza). Lyophilized. Contains PBS. Purity: >95% (SDS-PAGE). Mouse VISTA is a 55 to 65kDa type I Ig membrane protein with the extracellular domain homologous to PD-L1. VISTA is mainly expressed on hematopoietic tissues (spleen, thymus and bone marrow) and on myeloid cells with lower expression on CD4+ and CD8+ T cells. VISTA is a new negative checkpoint regulator that potently suppresses T cell activation. Overexpression of VISTA on tumors inhibits the protective anti-tumor immunity and blockade of VISTA enhances antitumor immunity in multiple tumor models. VISTA on APC delivers negative signaling to T cells via a yet-to-identify binding partner on T cells. Recently, it has been reported that recombinant VISTA protein needs to be multimerized to be active as soluble ligand. The protein VISTA (mouse):COMP (mouse) (with the extracellular domain of VISTA (mouse) fused to the pentamerization domain from the cartilage oligomeric matrix protein (COMP)), but not VISTA-Fc, functions as an immunosuppressive agonist in vivo inhibiting the proliferation of CD4+ T cells.
Gene ID: 74048
Swiss prot ID: Q9D659
Safety information: 
MSDS: MSDS
Additional information: 
Synonyms: 4632428N05Rik; Dies1; differentiation of ESCs 1; PD-1H; platelet receptor Gi24; VISTA; V-set domain-containing immunoregulatory receptor; V-set immunoregulatory receptor; V-type immunoglobulin domain-containing suppressor of T-cell activation
VISTA.COMP - an engineered checkpoint receptor agonist that potently suppresses T cell-mediated immune responses: A. Prodeus, et al.; JCI Insight 2, e94308 (2017) Read more