SeqPure PCR Purification System

Purified PCR Products That Are Ready-to-Use for Sequencing

For many molecular biology techniques, removing contaminants from DNA is critical for downstream applications. For example, to ensure a successful sequencing reaction (i.e. NGS), all excess contaminants such as primers, dNTPs, salts, and residual proteins, must be removed from the template. These agents can interfere with subsequent reactions and lead to unreadable sequences.

Biochain’s SeqPure PCR Purification System is designed for rapid and efficient purification of PCR products. The system utilizes paramagnetic bead technology to selectively bind DNA fragments 100 bp and larger in size. Simple washing steps will remove salts, primers, primer-dimers, nucleotides, and enzymes prior to elution of the purified PCR product. The SeqPure DNA Purification System is ideal for purification of DNA using either manual procedures or automated liquid handling instruments.

Features

Fast – purifies PCR products in under half an hour
Pure – removes salts, dNTPs, primers, and enzymes
Simple – no centrifugation or vacuum filtration needed
Ready-to-use – no fancy buffers; just use ethanol and Tris-HCl /TE
Economical – same performance as leading competitors at a more budget-friendly cost
Flexible – process between 1 to 384 samples simultaneously

Applications:

PCR
Genotyping
Sequencing, NGS
Cloning
Microarrays
Fragment analysis
Restriction enzyme digestion


1. SeqPure beads are added to the PCR product for DNA binding.		2. During the process, contaminants and salts are washed off.		3. Purified DNA is eluted, ready to be used in subsequent applications.

Excellent Binding Capability by SeqPure

Equal amounts of 100 bp ladder containing salts and proteins were purified with SeqPure beads and Ampure beads. Different buffers, TE and pre-warmed TE (for increasing the yield) were used for elution. Eluted DNA (8 µl each) were loaded and run on a 1% agarose gel. The gel shows excellent recovery of all fragments from 3000-100bp.

Figure 1. Equal amounts of 100 bp ladder containing salts and proteins were purified with SeqPure beads and Ampure beads. Different buffers, TE and pre-warmed TE (for increasing the yield) were used for elution. Eluted DNA (8 µl each) were loaded and run on a 1% agarose gel. The gel shows excellent recovery of all fragments from 3000-100bp.

Excellent purification and DNA recovery with the SeqPure magnetic bead system

Ratios Before Purification	A^260/280	A^260/230
100 bp ladder (w/ salts and proteins)	1.4	0.76

Ratios After Purification	A^260/280	A^260/230
SeqPure Beads	1.91	2.61
SeqPure Beads with pre-warmed TE	1.86	2.53
AmPure Beads	1.84	2.8
AmPure Beads with pre-warmed TE	1.84	2.65

Table 1. Comparison of SeqPure and AmPure in purifying 100 bp DNA ladder containing salts and protein. The purity was measured by spectrophotometry. Both the 260/280 and 260/230 absorbance ratios show that SeqPure is efficient at removing salts and proteins and is comparable to AmPure.

Efficient Recovery with SeqPure

Equal amounts of 100 bp ladder containing salts and proteins were purified with SeqPure beads and Ampure beads. Different buffer conditions, TE and pre-warmed TE (for increasing the yield) were used for elution. The SeqPure process showed better recovery of DNAs in both experimental conditions.

Figure 2. Equal amounts of 100 bp ladder containing salts and proteins were purified with SeqPure beads and Ampure beads. Different buffer conditions, TE and pre-warmed TE (for increasing the yield) were used for elution. The SeqPure process showed better recovery of DNAs in both experimental conditions.

Cat. No.	Description	Amount	Brand
Z1510001	SeqPure PCR Purification Kit	5 ml	BioChain
Z1510002	SeqPure PCR Purification Kit	50 ml	BioChain
Z1510003	SeqPure PCR Purification Kit	500 ml	BioChain

Related to:
Brands: BioChain
Product groups: Molecular Biology

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