Stem Cell Applications

A broad range of solutions for any Stem Cell Applications

Stem Cell Workflow

Stem cells research studies require in many cases to alter gene expression via overexpression or silencing. In this context, OZ Biosciences has developed a complete product line to transfect or transduce stem cells.




Figure 1: Mesenchymal Stem Cells (MSC) transduced with lentivus (MOI 1) and ViroMag R/L
 

Virus Applications

ViroMag Reagents are magnetic nanoparticles formulations designed to improve any virus infection or transduction capacities with the influence of a magnetic field. ViroMag R/L and AdenoMag reagents are optimized for use on Retrovirus/ Lentivirus and Adenovirus, respectively.
  • Highly enhance transduction efficiency with low vector doses
  • Accelerate the transduction process
  • Concentrate viruses onto cells in minutes
  • Synchronise cells adsorption/infection
  • Ideal for hard-to-infect and non-permissive cells
Viro-MICST Transduction Reagent, based on the Integrated Magnetic Immuno-Cell Sorting and Transduction (i-MICST™) Technology, allows to genetically modify cells directly on magnetic cell purification columns.



Figure 2: Percentage of Human Stem Cells (MSC, AdSC, AFSC, KG1a) transfected with DreamFect™ Stem reagent
 

DNA Transfection

DreamFect™ Stem, a biodegradable reagent, outperforms other transfection reagents on Stem Cells.
  • High transfection efficiency
  • Cell phenotype and differentiation potential are not affected
  • No toxicity due to reagent biodegradability and low DNA amount required
  • Reliable and reproducible results



Figure 3: Gene Silencing in GFP-AFSC using Lullaby Stem and siRNA targeting GFP

Figure 4: Transfection of mRNA encoding GFP with RmesFect Stem reagent
 

RNA Transfection

Lullaby™ Stem/ RmesFect™ Stem are specifically designed for siRNA/ mRNA transfection of Stem Cells.
  • High efficiency with reliable results
  • No toxicity due to their biodegradability and low siRNA/mRNA amount required
  • Lullaby Stem allows to reach up to 90% gene silencing
  • No need for nuclear uptake with RmesFect Stem, ideal for iPS generation. mRNA translation into proteins occurs in the cytoplasm. No genomic integration.



Figure 5: AdSC transfection with ProDeliverIN and R-Phycoerythrin
 

Protein Transfection

Pro-DeliverIN™ & Ab-DeliverIN™ transfection reagents are innovative reagents allowing intracellular delivery of biologically active proteins or antibodies.
  • Intracellular delivery of functional proteins and antibodies
  • Biodegradable and non-toxic
  • Perfect for any stem cells
  • Suitable for a variety of proteins including recombinant transcription factors
  • Fast protein delivery


Senescence Kit

Senescence Kit is ideal when cells in culture showed abnormalities typical of the Hayflick limit, after a long period of normal growth. The cells vary in size and shape, the cytoplasm begins to be granular with many cell inclusions and debris formed in the medium. Senescence goes with decreasing of proliferation potential, telomere shortening and impairment of cell function (i.e decrease of differentiation potential of stem cells). During senescence in mammalian cells, an endogenous lysosomal b-galactosidase is over-expressed and is accumulated within the aging cells. The presence of this b-Gal activity is a marker of aging cell population in vitro.
 OZ Biosciences Cellular Senescence Kit allows visualization of ageing cells and is:
  • Simple and Rapid
  • Ready-to-use
  • Economical



Source:
Brochure: Stem Cell Applications

Related to:
Brands: OZ Biosciences
Product groups: Assays, Molecular Biology