"Gelite TM X100 DNA Gel Stain 10,000X Water Solution "

Catalog number: 17700_100UL
Brand: AAT Bioquest
Packing: 100 ul
Price: € 91.00
Expected delivery time: 12 days
Quantity:
Comparison of DNA detection in 1% agarose gel in TBE buffer using Gelite™ Safe, EtBr, and SYBR® Safe
Two-fold serial dilutions of 1 kb D...
Summary of Ames test results
Ames mutagenicity test was performe...

Product specifications for - "Gelite TM X100 DNA Gel Stain 10,000X Water Solution "

Overview: 
Product group: Technique Specific Products
Category: Electrophoresis and Western blotting
Subcategory: Protein / DNA gel stain / ECL
Properties: 
UNSPSC: 41105300
Scientific information: 
Scientific info: AAT Bioquest is committed to designing our products to be environment-friendly. It is part of how we enable our customers to make the world healthier, cleaner, and safer. Ethidium bromide (EtBr) has been commonly used as a DNA stain for many years. However, EtBr is harmful if swallowed and is very toxic if inhaled. EtBr has been shown to be mutagenic in various tests and is an aquatic toxin. SYBR® Safe was introduced as a safer alternative to EtBr and SYBR® Green, but unfortunately, it is much less sensitive than SYBR® Green. It only has sensitivity comparable to EtBr. Gelite™ Safe has been developed specifically to be less hazardous than EtBr for staining DNA in agarose and acrylamide gels with much higher sensitivity. Gelite™ Safe has greatly improved safety and uncompromised sensitivity. The exceptional sensitivity and strong DNA binding affinity of Gelite™ Safe allows DNA to be stained prior to or post electrophoresis without destaining. In addition to its superior binding properties, Gelite™ Safe is essentially non-fluorescent in the absence of nucleic acids showing very low background fluorescence. Upon binding to nucleic acids, Gelite™ Safe exhibits a considerable fluorescence enhancement by several orders of magnitude greater than that of EtBr. Gelite™ Safe was optimized to be compatible with various instruments, including UV and blue-light transiluminators, gel documentation systems, and laser scanners. It is the first single formulation that can be used in either the green or red channel at your preference. Unlike the membrane-permeant SYBR® Green, which is highly toxic to cells and the environment, the membrane-impermeant properties of Gelite™ Safe make it a much safer and noncytotoxic alternative. Furthermore, Ames testing has confirmed Gelite™ Safe to be significantly less mutagenic than EtBr and SYBR® Green, even at concentrations well above the working concentration used for gel staining. Ames mutagenicity test was performed in a dose-dependent manner for all test dyes pretreated with an S9 fraction from rat liver (SYBR® is a trademark of ThermoFisher).