Hoechst 33258 [23491-44-3]

Catalog number: HY-15558_100mg
Brand: MedChem Express
Packing: 100 mg
Other sizes: 50 mg
10 mM
Price: € 131.00
Expected delivery time: 10 days

Product specifications for - Hoechst 33258 [23491-44-3]

Product group: Technique Specific Products
Category: Immunohistochemistry
Subcategory: DAPI / Hoechst / Hematoxylin
CAS No.: 23491-44-3
Purity: >98%
Molecular Formula: C25H24N6O
Molecular weight: 424.4977
Datasheet: Datasheet
  Research Use Only
UNSPSC: 41116134
Scientific information: 
Scientific info: Hoechst stains are part of a family of blue fluorescent dyes used to stain DNA. Hoechst 33258 is a cell dye for DNA quantitation. IC50 Value: These Bis-benzimides were originally developed by Hoechst AG, which numbered all their compounds so that the dye Hoechst 33342 is the 33342nd compound made by the company. There are three related Hoechst stains: Hoechst 33258, Hoechst 33342, and Hoechst 34580. The dyes Hoechst 33258 and Hoechst 33342 are the ones most commonly used and they have similarexcitation/emission spectra. Both dyes are excited by ultraviolet light at around 350 nm, and both emit blue/cyan fluorescent light around anemission maximum at 461 nm. Unbound dye has its maximum fluorescence emission in the 510-540 nm range. Hoechst dyes are soluble in water and in organic solvents such as dimethyl formamide or dimethyl sulfoxide. Concentrations can be achieved of up to 10 mg/mL. Aqueous solutions are stable at 2-6 °C for at least six months when protected from light. For long-term storage the solutions are instead frozen at <=-20 °C. The dyes bind to the minor groove of double-stranded DNA with a preference for sequences rich in adenine andthymine. Although the dyes can bind to all nucleic acids, AT-rich double-stranded DNA strands enhance fluorescence considerably. Hoechst dyes are cell-permeable and can bind to DNA in live or fixed cells. Therefore, these stains are often called supravital, which means that cells survive a treatment with these compounds. Cells that express specific ATP-binding cassette transporter proteins can also actively transport these stains out of their cytoplasm.
Safety information: 
Additional information: 
Synonyms: HY-15558; bisBenzimide H 33258; H 33258; Hoechst33258; Hoechst-33258
Methylene blue increases the amount of HSF1 through promotion of PKA-mediated increase in HSF1-p300 interaction.', Chao Huang. Int J Biochem Cell Biol. 2017 Jan 16;84:75-88. Read more