Hoechst 33258 trihydrochloride [23491-45-4]

Catalog number: HY-15558A_100mg
Brand: MedChem Express
Packing: 100 mg
Other sizes: 50 mg
10 mM
Price: € 131.00
Expected delivery time: 10 days
Quantity:

Product specifications for - Hoechst 33258 trihydrochloride [23491-45-4]

Overview: 
Product group: Technique Specific Products
Category: Immunohistochemistry
Subcategory: DAPI / Hoechst / Hematoxylin
CAS No.: 23491-45-4
Properties: 
Molecular Formula: C25H27Cl3N6O
Molecular weight: 533.88048
Datasheet: Datasheet
  Research Use Only
UNSPSC: 41116134
Scientific information: 
Scientific info: Hoechst 33258 trihydrochloride is a fluorescent dyes, which can be used as a cell dye for DNA. IC50 & Target: IC50: 51.31+/-4.56 uM (HeLa cell), 32.43+/-3.27 uM (HL60 cell), 15.42+/-2.16 uM (U937 cell)[1] In Vitro: Hoechst 33258, a fluorescent compound with a head-to-tail bis-benzimidazole structure, is initially found to be cytotoxic against L1210 murine leukemia. Hoechst 33258 is evaluated for their cytotoxicity against human tumor cell lines, which are cervix carcinoma cell line (HeLa), Human promyelocytic leukemia cell (HL60) and U937 cell Line. The IC50 determined in the case of HeLa, HL60 and U937 is 51.31+/-4.56, 32.43+/-3.27 and 15.42+/-2.16 uM for Hoechst 33258, respectively[1]. The cytotoxic property of Hoechst 33258 is investigated on a panel of seven tumour cell lines of different histological origin and Madine-Darby canine kidney (MDCK) normal cells. All cell lines, except MCF-7, exposed to Hoechst 33258 exhibit GI50 from 84x10-6 to 191.5x10-6 mol/dm3. Under the same experimental conditions, Hoechst 33258, used as a binder reference compound, stops the cell cycle in S phase and G0/G1[2].
Safety information: 
MSDS: MSDS
Additional information: 
Synonyms: HY-15558A