ChIPAb+ E2F-1

Catalog number: 17-10061
Brand: Merck
Packing: 25 assays
Price: € 431.00
Expected delivery time: 7 days

Product specifications for - ChIPAb+ E2F-1

Product group: Antibodies
Category: Primary Antibodies
Application: ChIP
Application note: Chromatin Immunoprecipitation: Representative lot data.Sonicated chromatin prepared from HeLa cells (1 X 10E6 cell equivalents per IP) were subjected to chromatin immunoprecipitation using 1 uL of either Normal Mouse IgG or 1 uL of Anti-E2F-1 and the Magna ChIP G Kit (Cat. # 17-611). Successful immunoprecipitation of E2F-1-associated DNA fragments was verified by qPCR using ChIP Primers, human CDC2 promoter as a positive locus, and an intergenic region near human CALML5 as a negative locus. (Please s
Species: Human, Xenopus
Host: Mouse
Clonality: Monoclonal
  Research Use Only
UNSPSC: 12352203
Storage instructions: Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Scientific information: 
Scientific info: E2F-1 was identified as a cellular protein with DNA binding activity associated with the adenovirus E2 gene promoter. The original member of the E2F family of transcription factors, now consisting of five, was identified through its association with the retinoblastoma protein, which controls its activity. E2F-1 is cell cycle regulated with very low levels in early G1 then increasing levels as cells move from G1 to S with highest levels of protein at the G1/S boundary, which is consistent with its role in S-phase entry.
Additional information: 
Synonyms: 17-10061; Merck; Millipore