DAP Kinase 1 (phospho Ser308) antibody [DKPS308]
GTX10524
ApplicationsImmunoPrecipitation, Western Blot, ELISA
Product group Antibodies
ReactivityHuman
Overview
- SupplierGeneTex
- Product NameDAP Kinase 1 (phospho Ser308) antibody [DKPS308]
- Delivery Days Customer10
- ApplicationsImmunoPrecipitation, Western Blot, ELISA
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDDKPS308
- HostMouse
- IsotypeIgG
- Modification TypePhosphorylated
- Scientific DescriptionVarious apoptotic signals use Death Associated Protein Kinase (DAPK) as a downstream effector in different cell types. DAPK is a positive mediator of apoptosis and is widely expressed in many tissues of embryonic and adult origin. DAPK is a Ca2+/calmodulin dependent Ser/Thr kinase that associates with microfilaments. The protein is composed of a multidomain structure. It has a subdomain typical of serine/threonine kinases, a Ca2+/calmodulin regulatory domain, eight ankyrin repeats followed by two P-loop motifs and a typical death domain module. It contains two auto-inhibitory domains one of them Ca2+/calmodulin dependent. In the absence of this latter domain, DAPK is constitutively active. DAPK activity is also regulated by phosphorylation. DAPK was found to be negatively regulated by autophosphorylation at Ser308 which is in the calmodulin regulatory domain. This autophosphorylation, which occurs in cells at the basal state, lowers the affinity of DAPK for calmodulin and thus the kinase is inactive. Under some apoptotic conditions DAPK undergoes dephosphorylation. As a consequence, it binds to calmodulin with higher affinity, becomes activated, phosphorylates its downstream substrate proteins, and mediates apoptosis. Monoclonal antibodies specific for Phospho DAP-Kinase (pSer308) are important tools for studying the mechanism of DAPK activation in apoptosis.
- ReactivityHuman
- Storage Instruction2°C to 8°C,-20°C
- UNSPSC12352203
References
- Eburicoic Acid, an Active Triterpenoid from the Fruiting Bodies of Basswood Cultivated Antrodia cinnamomea, Induces ER Stress-Mediated Autophagy in Human Hepatoma Cells. Su YC et al., 2012 Oct, J Tradit Complement MedRead more