Human RSPO3 / R-spondin 3 (aa 1-146) Human Cells Transfected Lysate (positive control) (denatured)

Catalog number: 11185-H08H1L
Brand: Sino Biological
Packing: 300 ug
Price: € 285.00
Expected delivery time: 10 days
Quantity:

Product specifications for - Human RSPO3 / R-spondin 3 (aa 1-146) Human Cells Transfected Lysate (positive control) (denatured)

Overview: 
Product group: Lysates / Extracts
Category: Lysates
Application: Western Blot
Application note: WB: Use at an assay dependent dilution.Not yet tested in other applications.Optimal dilutions/concentrations should be determined by the end user.
Species: Human
Target: RSPO3
Target description: R-spondin 3
Properties: 
Datasheet: Datasheet
  Research Use Only
UNSPSC: 41105330
Storage instructions: Store at -80-¦C. Aliquot to avoid repeated freezing and thawing
Scientific information: 
Scientific info: Human Cells transfected lysate in which Human RSPO3 / R-spondin 3 (aa 1-146) has been over-expressed. The whole cell lysate is provided in 1X Sample Buffer. Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized; Stability: Samples are stable for up to twelve months from date of receipt at -80°C
Gene ID: 84870
Additional information: 
Synonyms: 11185-H08H1L-300; RSPO3; THSD2; PWTSR; CRISTIN1; thrombospondin, type I, domain containing 2; thrombospondin type-1 domain-containing protein 2; roof plate-specific spondin-3; protein with TSP type-1 repeat; R-spondin-3; R-spondin 3 homolog; R-spondin 3