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ProductsBack/DAPI . dihydrochloride [28718-90-3]
Chemical Structure
Chemical Structure
Chemical Structure

DAPI . dihydrochloride [28718-90-3]

Research Use Only
AG-CR1-3668
AdipoGen Life Sciences
Estimated Purity>97% (HPLC)
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Product group Chemicals
Molecular Weight277.3 . 72.9
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Overview

  • Supplier
    AdipoGen Life Sciences
  • Product Name
    DAPI . dihydrochloride [28718-90-3]
  • Delivery Days Customer
    10
  • Certification
    Research Use Only
  • Estimated Purity
    >97% (HPLC)
  • Hazard Information
    Non-hazardous,Warning
  • Molecular Formula
    C16H15N5 . 2HCl
  • Molecular Weight
    277.3 . 72.9
  • Scientific Description
    Cell permeable fluorescent DNA stain. AT-sequence-specific DNA intercalator. Binds to the minor groove of double-stranded DNA (preferentially to adenine and thymine (AT) rich DNA), like Hoechst dyes, forming a stable complex which fluoresces approximately 20 times greater than DAPI alone. Spectral Data: Excitation: lambdaex 340 nm; Emission: lambdaem 488 nm (only DAPI). Excitation: lambdaex 360nm; Emission: lambdaem 460nm (DAPI-DNA complex). Commonly used to stain DNA and chromosomes for fluorescent microscopy and flow cytometry applications. DAPI is often used as a counterstain, as its ultraviolet excitation (lambdaex 360nm) and blue emission (lambdaem 460nm) wavelengths separate it nicely from many popular primary fluorophores. Can be used on either fixed or live cells, although it passes through the membrane less efficiently in live cells and therefore the effectiveness of the stain is lower and demands higher concentrations to be used. Reversible inhibitor of S-adenosyl-L-methionine decarboxylase and KAO (diamine oxidase). - Chemical. CAS: 28718-90-3. Formula: C16H15N5 . 2HCl. MW: 277.3 . 72.9. Synthetic. Cell permeable fluorescent DNA stain. AT-sequence-specific DNA intercalator. Binds to the minor groove of double-stranded DNA (preferentially to adenine and thymine (AT) rich DNA), like Hoechst dyes, forming a stable complex which fluoresces approximately 20 times greater than DAPI alone. Spectral Data: Excitation: lambdaex 340 nm; Emission: lambdaem 488 nm (only DAPI). Excitation: lambdaex 360nm; Emission: lambdaem 460nm (DAPI-DNA complex). Commonly used to stain DNA and chromosomes for fluorescent microscopy and flow cytometry applications. DAPI is often used as a counterstain, as its ultraviolet excitation (lambdaex 360nm) and blue emission (lambdaem 460nm) wavelengths separate it nicely from many popular primary fluorophores. Can be used on either fixed or live cells, although it passes through the membrane less efficiently in live cells and therefore the effectiveness of the stain is lower and demands higher concentrations to be used. Reversible inhibitor of S-adenosyl-L-methionine decarboxylase and KAO (diamine oxidase).
  • SMILES
    [H]Cl.[H]Cl.NC(C1=CC=C2C(NC(C3=CC=C(C(N)=N)C=C3)=C2)=C1)=N
  • Storage Instruction
    2°C to 8°C,-20°C
  • UNSPSC
    12352200

References

  • A simple cytochemical technique for demonstration of DNA in cells infected with mycoplasmas and viruses: W.C. Russell, et al.; Nature 253, 461 (1975)
  • DIPI and DAPI: fluorescence banding with only negliglible fading: W. Schnedl, et al.; Hum. Genet. 36, 167 (1977)
  • An evaluation of DNA fluorochromes, staining techniques, and analysis for flow cytometry. I. Unperturbed cell populations: I.W. Taylor & B.K. Milthorpe; J. Histochem. Cytochem. 28, 1224 (1980)
  • A comparative study of DAPI, DIPI, and Hoechst 33258 and 33342 as chromosomal DNA stains: F. Otto & K.C. Tsou; Stain Technol. 60, 7 (1985)
  • Estimation of total DNA in crude extracts of plant leaf tissue using 4',6-diamidino-2-phenylindole (DAPI) fluorometry: L.S. Lee & H.M. Garnett; J. Biochem. Biophys. Methods 26, 249 (1993)
  • 4',6-Diamidino 2-phenylindole is a new reversible inhibitor of diamine oxidase and S-adenosyl-L-methionine decarboxylase from mammalian tissues: C. Cubria, et al.; Comp. Biochem. Physiol. C. 105, 251 (1994)
  • Flow cytometric detection of apoptosis: comparison of the assays of in situ DNA degradation and chromatin changes: M.A. Hotz, et al.; Cytometry 15, 237 (1994)
  • DAPI: a DNA-specific fluorescent probe: J. Kapuscinski; Biotech. Histochem. 70, 220 (1995)
  • The use of DAPI as a replacement for ethidium bromide in forensic DNA analysis: E. Buel & M. Schwartz; J. Forensic Sci. 40, 275 (1995)
  • Visualizing chromatin and chromosomes in living cells: D. Zink, et al.; Methods: A Companion to Methods in Enzymology 29, 42 (2003)