JBS Solubility Kit

Jena Bioscience

JBS Solubility Kit

5

Research Use Only

Since the highly complex properties of proteins are dependent on their environment, buffer solutions play an important role, i.e. influencing the solubility and the aggregation behaviour of the protein sample. Studies have shown that aggregation of the protein may inhibit nucleation and crystal growth. Therefore, the JBS Solubility Kit has been designed to investigate protein samples towards their homogeneity and monodispersity in dependence of the buffer solution, employing hanging-drop vapour diffusion experiments combined with dynamic light scattering. Composition: The JBS Solubility Kit comprises two individual kits for successive use: A: Buffer Kit 24 buffer solutions at different pH values, 100 mM, supplied in 10 ml volumes No. - - Buffer - - pH 1 - - Glycine - - 3.0 2 - - Sodium Citrate - - 3.2 3 - - PIPPS - - 3.7 4 - - Sodium Citrate - - 4.0 5 - - Sodium Acetate - - 4.5 6 - - Sodium / Potassium Phosphate - - 5.0 7 - - Sodium Citrate - - 5.5 8 - - Sodium / Potassium Phosphate - - 6.0 9 - - Bis-Tris - - 6.0 10 - - MES - - 6.2 11 - - ADA - - 6.5 12 - - Bis-Tris Propane - - 6.5 13 - - Ammonium Acetate - - 7.0 14 - - MOPS - - 7.0 15 - - Sodium / Potassium Phosphate - - 7.0 16 - - HEPES - - 7.5 17 - - Tris - - 7.5 18 - - EPPS - - 8.0 19 - - Imidazole - - 8.0 20 - - Bicine - - 8.5 21 - - Tris - - 8.5 22 - - CHES - - 9.0 23 - - CHES - - 9.5 24 - - CAPS - - 10.0 B: Additive Kit 14 Additives solutions, supplied in 250 microl volumes, ready to use, concentration adjusted No. - - Additive - - Concentration of Stock Solution 1 - - Sodium Chloride - - 80 mM 2 - - Sodium Chloride - - 200 mM 3 - - Sodium Chloride - - 400 mM 4 - - Glycerol - - 20% 5 - - Glycerol - - 40% 6 - - CHAPS - - 8 mM 7 - - Octyl Glucopyranoside - - 0.4 % 8 - - Octyl Glucopyranoside - - 4% 9 - - Dodecyl Maltoside - - 0.4 % 10 - - Dodecyl Maltoside - - 4% 11 - - BME - - 40 mM 12 - - DTT - - 4 mM 13 - - DTT - - 20 mM 14 - - TCEP - - 120 mM

2°C to 8°C

12352200