Animal-Origin-Free Recombinant Proteins for R&D and Manufacturing
Provis Biolabs manufactures and supplies animal-origin-free recombinant enzymes and proteins designed to support reproducible, scalable and reliable manufacturing of biologics, vaccines and advanced therapies.
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Find products for your researchThe development and manufacturing of modern biotechnological products increasingly rely on recombinant, animal-origin-free raw materials to support process consistency, biosafety and control of adventitious agents. Recombinant enzymes and proteins play a critical role across bioprocessing, vaccines, peptide therapeutics, diagnostics and cell & gene therapy workflows.
Provis Biolabs offers a focused portfolio of animal-origin-free recombinant proteins and enzymes, available in both Research Use Only (RUO) and GMP grades, enabling a structured transition from early research to commercial manufacturing.
Recombinant Trypsin for Cell Culture and Bioprocessing
Recombinant Trypsin is widely used for cell detachment in adherent cell culture systems and protein processing such as in insulin manufacturing. Compared to animal-derived trypsin, recombinant trypsin offers improved batch-to-batch consistency and reduces risks associated with animal-origin impurities and hence supports regulatory requirements.
Recombinant Supplements for Cell Culture Media
Serum-derived supplements can introduce variability and contamination risks. Recombinant alternatives provide greater control and reproducibility in cell culture applications.
| Product | Primary Function | Typical Applications |
| Recombinant Human Albumin (rAlbumin) | Protein stabilisation and nutrient transport | Cell culture media, biologics formulation, vaccines |
| Recombinant Transferrin (rTransferrin) | Iron transport | Serum replacement, media supplementation |
| Recombinant Insulin (rInsulin) | Cell growth and metabolism | mAbs, viral vaccines, stem cell expansion |
Recombinant human albumin enhances media stability and nutrient availability, improving cell health and reproducibility in serum-free and animal-origin-free processes.
Recombinant transferrin ensures efficient iron delivery to support rapid cell growth and reliable performance in serum-replacement formulations.
Recombinant insulin promotes sustained cell growth and productivity, helping maintain consistent upstream performance over extended culture durations.
Proteolytic Enzymes for Insulin and Peptide Manufacturing
Recombinant proteases are essential for the controlled manufacturing of therapeutic peptides, including insulin and GLP-1 analogues such as Semaglutide.
| Enzyme | Function | Manufacturing Use |
| Enterokinase | Site-specific cleavage | Pro-protein activation |
| Carboxypeptidase B | C-terminal processing | Insulin maturation and antibody processing |
| Kex2 Protease | Pro-peptide cleavage | Fusion Protein Processing |
Provinase® Endonuclease for Viral Vector and Cell & Gene Therapy
Efficient reduction of residual host-cell DNA is critical in viral vector manufacturing and cell & gene therapy workflows. Provinase®, a recombinant endonuclease derived from Serratia marcescens, enables effective nucleic acid digestion during downstream processing.
Provinase® – Application Scope
| Application Area | Function |
| AAV manufacturing | Reduction of residual host-cell DNA |
| Lentiviral vectors | Improved downstream purification |
| Cell & gene therapy | Supports DNA clearance strategies |
PNGase F for Glycoprotein Analysis and Characterisation
PNGase F is an animal-origin-free recombinant enzyme used for the enzymatic removal of N-linked glycans from glycoproteins. It is widely applied in protein characterisation, comparability studies and quality assessment workflows, where understanding glycosylation patterns is critical. PNGase F supports analytical methods such as SDS-PAGE, mass spectrometry and chromatography by enabling accurate evaluation of protein structure and glycan occupancy in biologics and recombinant protein development.
Recombinant Streptavidin for Biotin-Based Applications
Recombinant Streptavidin is an animal-origin-free protein widely used in biotin–streptavidin systems for high-affinity binding applications. It supports protein capture, detection and purification workflows across research and biomanufacturing, including affinity chromatography, immunoassays, and diagnostic platforms. Recombinant production ensures consistent binding performance and reduced variability compared to animal-derived sources.
RUO and GMP Grades to Support Development Pathways
Provis Biolabs products are available in both RUO and GMP grades, supporting consistent raw-material selection throughout development.
RUO vs GMP – Comparison
| Attribute | RUO Grade | GMP Grade |
| Intended use | Research & process development | Clinical & commercial manufacturing |
| Manufacturing environment | Controlled and non-GMP | ISO-certified clean rooms and GMP-compliant |
| Documentation | Datasheet and CoA | CoA, CoC, certifications, vendor qualification and related quality documentation |
| Change control | Limited | Formal change control and quality management systems |
| Compliance | Limited such as internal standards and ISO 9001-2015 | Relevant regulatory certifications such as ISO, GMP, Halal and Kosher |
CDMO Services
In addition to its animal-origin-free recombinant product portfolio, Provis Biolabs provides custom development services supporting recombinant proteins and enzymes from early research through manufacturing readiness. These services include construct design, expression optimisation and process development using microbial, yeast and mammalian expression systems, with integrated upstream and downstream capabilities to support scalable biomanufacturing workflows.
Conclusion
By offering animal-origin-free recombinant enzymes and proteins across cell culture, bioprocessing, vaccines, biologics, peptide therapeutics and viral vector manufacturing, Provis Biolabs supports reliable and sustainable research, scalable manufacturing and consistent product quality from early development through commercial production.
