ELISpot assay principle
Mabtech: The enzyme-linked immunospot (ELISpot) assay is an extremely sensitive immunoassay performed in a 96-well plate format to quantify protein secreting cells.
With 25 years of experience and a dedicated team, U-CyTech specializes in cytokine ELISA, B cell and T cell ELISPOT assays, FluoroSpot kits and monoclonal antibodies.
U-CyTech biosciences has many cytokine ELISA and ELISPOT assays and corresponding antibodies from human and various animal species. IL-21 is a cytokine of interest.
IL-21 is a pro-inflammatory cytokine and is produced by several T cells, including CD4+, T follicular helper and T-helper 17 cells. IL-21 supports both cytotoxic and humoral arms of the immune response.
Overproduction of IL-21 occurs in many inflammatory diseases such as rheumatoid arthritis, SLE, organ transplantation and Covid.
The level of IL-21 in supernatant, serum or plasma can be determined by ELISA. The IL-21 capacity of T cells or the frequency of IL-21 producing cells can be determined by ELISPOT assay.
With the Human IL-21 ELISA or ELISPOT kit from U-CyTech biosciences, you can determine the important IL-21 cytokine.
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