Atlas Antibodies’ AtlasPlex delivers low-plex multiplex immunofluorescence in a single day. Choose your targets, pick your kit size, and get all core components included in one click. Simple, fast, and fully customizable.
Multiplex immunofluorescence (mIF) has transformed tissue-based proteomics, allowing researchers to visualize multiple biomarkers simultaneously in a single section. It has become essential in oncology, spatial biology, and biomarker discovery because it preserves spatial context while increasing experimental throughput.
However, current mIF approaches face significant challenges:
High technical barriers (optimization often requires specialized expertise and infrastructure)
For many studies in research labs, especially those focusing on low-plex (3–5 targets) investigations, these limitations slow progress and introduce avoidable complexity.
AtlasPlex: A Faster, More Flexible, Streamlined Approach
AtlasPlex is a multiplex immunofluorescence kit, designed and manufactured by Atlas Antibodies, that addresses and resolves common mIF challenges head-on.
AtlasPlex is a ready-to-use multiplexing assay designed to simplify and accelerate biomarker discovery. This all-inclusive kit combines a pre-validated TSA (Tyramide Signal Amplification) system with 3 or 5 pre-biotinylated primary antibodies and tyramide dyes, thus eliminating the need for secondary antibodies and offering unmatched flexibility for tissue multiplexing, especially in FFPE (formalin-fixed, paraffin-embedded) samples.
3-plex or 5-plex formats: optimized to reduce spectral overlap and preserve signal intensity.
No fixed panels, complete flexibility: over 12,000 validated antibodies to choose from.
TSA amplification: boosts sensitivity for detecting low-abundance proteins.
No secondary antibodies: pre-biotinylated primaries simplify the workflow.
With AtlasPlex, you bypass the usual hurdles of manual antibody conjugation and TSA optimization. Choose any target from the wide Atlas Antibodies’ catalog (over 12,000 validated targets) and get started immediately with a straightforward yet powerful workflow, without sacrificing clarity or reliability.
Figure 1 AtlasPlex enables iterative multiplexed immunohistochemistry (mIHC) for 3-5 proteins detection in FFPE tissue.
Smart Complexing (step 1) Biotinylated primary antibodies (Ab1) are pre-bound to StreptaClick®-HRP, setting the stage for powerful signal amplification.
Targeted Tissue Binding (step 2) Apply the Ab1-HRP complex directly to fixed tissue. Binding is precise, reliable, and reproducible.
Amplification (step 3) Tyramide-based signal amplification deposits a bright, permanent fluorescent signal at each target site, ensuring crisp, high-contrast images.
Enzyme Quenching (step 4) Deactivate HRP quickly and cleanly. No interference in downstream rounds.
Repeat (↻) Run multiple antibody cycles (Ab2…Ab5) with only 1.5 hours per cycle. Up to 5-plex per day.
Brilliant Imaging (step 5) Capture your results using standard fluorescence microscopy.
HRP Labeling & Quenching (Core Components. No selection needed)
Tyramide Dyes (Core Components. No selection needed)
AtlasPlex: Your Needs.Your Targets.
AtlasPlex puts you in complete control of your multiplex immunofluorescence experiments.
Drawing on Atlas Antibodies’ unique legacy as a provider of enhanced-validated antibodies, the platform offers one of the most extensive catalogs available for low-plex customization.
You’re free to design a panel that precisely reflects your research hypothesis, without being constrained by rigid, pre-selected marker sets.
The technology is meticulously optimized for FFPE tissue sections, guaranteeing seamless integration with standard histopathology workflows while delivering publication-ready results.
With AtlasPlex, you’re not just running assays, you’re building on a foundation of scientific excellence, backed by decades of antibodies expertise.
One click purchase: How to Customize your AtlasPlex Kit
The AtlasPlex kit configurator is built for simplicity and speed. You only need to choose two things: your targets (up to 5 from 12,000+ validated options) and your kit size (3-plex or 5-plex)
All other components (i.e. pre-biotinylated antibodies, TSA amplification reagents, and workflow materials) are core inclusions.
No extra decisions, no hidden add-ons. Just select your essentials, click “order,” and your complete kit is on its way.
Select Your Targets
Build Your Own Antibody Panel. AtlasPlex allows you to design low-plex (3-plex or 5-plex) multiplex immunofluorescence panels using any combination of antibodies from Atlas Antibodies’ extensive catalog — over 12,000 validated antibodies. You’re not limited to preset marker panels and can tailor the kit precisely to your experimental needs.
One-Click Online Ordering
The process is streamlined: browse the online catalog, choose your targets, specify your desired format (3-plex or 5-plex), and place your order — typically in a single click. This triggers the preparation of a custom kit assembled just for your panel.
Receive a Complete, Ready-to-Use Kit
The ordered kit includes pre-biotinylated primary antibodies, TSA amplification reagents, and all necessary instructions. Everything arrives quality-checked and prepared for immediate use, no additional reagent assembly or validation required.
Run your experiment: From Sample to Results Within One Day
The kit is optimized for a same-day workflow: from FFPE tissue section to multiplexed imaging in just one working day. The pre-validated reagents and TSA amplification ensure high sensitivity and clear, reproducible results.
By streamlining panel design, reagent preparation, and staining, AtlasPlex minimizes downtime and maximizes data acquisition.
Scientific Insight: Robust Multiplexing for Proteomics and Beyond
Developed to meet the evolving researchers need of fast and streamelined daily multiplexed tissue analysis, AtlasPlex comes from the Atlas Antibodies team, emerging from the Human Protein Atlas project, with a deep legacy in antibody manufacturing, validation, and quality.
With over 22,000 primary antibodies, Atlas Antibodies ensures robust performance in: immunohistochemistry (IHC), immunocytochemistry (ICC), western blot (WB) and chromatin immunoprecipitation (ChIP).
Every antibody is backed by rigorous validation to ensure high specificity, reproducibility, and consistent performance across applications.
Contact your Bio-Connect account manager for more information.
AtlasPlex is more than just a multiplex kit. It’s an innovation in proteomics and antibody multiplexing. By fusing a simplified workflow with expansive antibody choices and powerful TSA amplification, AtlasPlex empowers you to:
Build custom, low-plex panels.
Skip species-dependent challenges and secondary antibody steps.
Achieve clearer signals and faster discoveries in tissue-based multiplex immunofluorescence.
Elevate your multiplexing proteomics research today.
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