OriGene Advances Gene Delivery With ProteanFect™

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A New Solution for Hard-to-Transfect Cells

OriGene Technologies has introduced ProteanFect™, a groundbreaking coacervate-based gene delivery reagent designed to redefine transfection performance across a broad range of cell systems. The technology represents one of the most significant innovations in nucleic acid delivery in recent years.

A key strength of ProteanFect™ is its ability to perform exceptionally well in hard-to-transfect cell types, a long-standing challenge in molecular and cell biology research. These include:

• Primary immune cells (e.g., T-cells, NK cells)
• Neuronal cells and iPSC-derived neurons
• Stem cells and progenitor cells
• Suspension cell lines such as Jurkat
• Delicate or low-viability primary cultures

Successfully Transfected Cell Types

Find here the Full Lists of Successfully Transfected Cell Types

Engineered for improved Efficiency and reduced Cytotoxicity

Unlike conventional lipid- or polymer-based transfection reagents, ProteanFect™ leverages mammalian endogenous proteins to form dynamic coacervate complexes capable of encapsulating and delivering plasmid DNA (pDNA), mRNA, and other nucleic acid cargos with unparalleled precision. This biologically inspired mechanism mirrors natural intracellular pathways, resulting in superior biocompatibility, reduced cytotoxicity, and highly efficient gene expression outcomes.

Proof is in the data…

The scientific foundation of ProteanFect™ aligns with a rapidly growing body of literature describing the role of liquid–liquid phase separation and biomolecular condensates in cellular organization. Notable publications include:

• Banani et al., 2017, Nature Reviews Molecular Cell Biology — describing condensates as functional cellular compartments.
• Shin & Brangwynne, 2017, Science — detailing biophysical principles governing intracellular phase separation.
• Alshareedah et al., 2021, nature communications — demonstrating how condensates encapsulate and transport biomolecules.

With its innovative mechanism, strong performance characteristics, and broad cell-type applicability, ProteanFect™ is positioned to become a next-generation standard for nucleic acid delivery. OriGene continues to expand its portfolio of advanced research tools, and ProteanFect™ stands out as a transformative solution for laboratories striving to overcome the most persistent barriers in gene delivery.

Figure 1. ProteanFect achieves high transfection efficiency with pDNA/mRNA in Jurkat T Cells.

Figure 2. ProteanFect achieves high transfection efficiency with mRNA in CD34+ hematopoietic stem cells.

Would you like to learn more about ProteanFect™? Please feel free to contact us!