Stop Mycoplasma Contamination with abm’s MycOut™

Why Mycoplasma Contamination Threatens Your Cell Culture

Mycoplasma contamination is one of the most persistent and disruptive problems in cell culture. The lack of a cell wall in mycoplasmas makes them resistant to many common antibiotics, while their small size allows them to pass through standard filtration systems undetected. Infected cultures often show no visible turbidity, yet suffer altered gene expression, disrupted metabolism, reduced growth rates, and unreliable experimental outcomes.
For researchers, that means unreliable data, unsafe biologics, and wasted resources. Because mycoplasmas rarely cause visible turbidity, specialized detection methods such as PCR are necessary to confirm their presence. Effective mycoplasma control requires a dedicated workflow of prevention, detection, and elimination.

abm’s Complete Mycoplasma Toolbox

Applied Biological Materials (abm) offers a comprehensive, simple, effective defence for every stage of mycoplasma control. Whether you are protecting healthy cultures or rescuing contaminated ones, abm’s antimicrobial agents and PCR detection kits deliver reliable, reproducible results.

MycOut™ 1000X Culture Guard: Prevention & Elimination

MycOut™ (Cat. No. G7001) is a simple, effective defence against mycoplasma. Its dual-action mechanism and low-toxicity formulation make it ideal as an easy add-on to routine cell culture workflows.
Used at a 1:2000 dilution for prevention, MycOut™ demonstrated better mycoplasma clearance than Penicillin/Streptomycin and the leading competitor product, confirmed by microscopy and by abm’s Mycoplasma PCR Detection Kit. Following two weeks of MycOut™ treatment and passaging, contaminated BeWo cells, JHH7 cells, immortalized human ependymoma cells, and immortalized bovine myoblasts displayed improved growth and restored cell morphology compared to untreated cultures.
Try it free: abm offers a complimentary MycOut™ sample (Cat. No. Sample-G7001, 250 µl) so you can validate performance in your own lab.

MycoAway™  For MycOut™-Resistant Strains

Reliable prevention and elimination start with reliable detection. abm’s detection portfolio includes:

• Mycoplasma PCR Detection Kit (G238): standard endpoint PCR, 100 reactions
• Mycoplasma Pro PCR Detection Kit (G239): endpoint PCR, 100 reactions
• Mycoplasma qPCR Detection Kit (G240): quantitative real-time PCR

A Complete Workflow in Under 2 Weeks

abm’s recommended protocol is straightforward: add MycOut™ or MycoAway™ to your culture, perform four passages over two weeks with fresh treatment-containing medium, and confirm clearance with a mycoplasma detection test. If negative, retest every 2-3 weeks. If positive, continue treatment and retest weekly.

Order Through Bio-Connect

As the official BeNeLux distributor for abm, Bio-Connect provides fast local delivery, expert technical support, and access to the full abm mycoplasma portfolio. Contact us to request your free MycOut™ sample or to discuss the best solution for your lab.