Detect immune responses with ELISpot and Mabtech IRIS 2

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High-sensitivity immune cell detection with ELISpot & FluoroSpot on IRIS 2

Mabtech’s ELISpot and FluoroSpot assays are trusted tools for detecting and quantifying cytokine-secreting cells at the single-cell level, essential in immunology, infectious disease, vaccine development, and cancer research. When paired with the Mabtech reader, labs benefit from fully automated, high-throughput imaging, and precise spot analysis.

Why ELISpot and FluoroSpot?

The ELISpot technique enables researchers to visualize cytokine secretion (such as IFN-γ, IL-2, IL-4, and more) from individual immune cells. FluoroSpot builds on this by enabling multiplex detection, identifying cells that produce multiple cytokines simultaneously, delivering deeper insight into immune function.

IRIS 2: Automation meets accuracy

The Mabtech IRIS 2 reader simplifies and accelerates spot detection with its Apex software, offering fast, reliable image capture, and advanced spot-counting algorithms. Whether you’re running single-color ELISpot or multi-color FluoroSpot assays, IRIS 2 adapts seamlessly.

Key benefits include:

• Automated plate scanning and analysis
• Robust algorithms for both low- and high-intensity spot detection
• Support for multiplex fluorescence in FluoroSpot assays
• Easy setup and reproducible results, even across large studies

Applications in immune monitoring

• Vaccine research – Measure T-cell and B-cell responses to antigen exposure
• Immunotherapy – Monitor cytokine profiles in response to treatment
• Infectious disease – Study host immune response at the cellular level
• Transplantation – Assess rejection risk and immune activation

With streamlined protocols, highly sensitive detection, and IRIS 2 automation, Mabtech’s ELISpot and FluoroSpot kits provide researchers with unmatched flexibility and confidence in immune monitoring.