High-Affinity Recombinant Streptavidin for Biotin-Based Assays

23 October 2026

Provis Biolabs recombinant Streptavidin is a high-affinity biotin-binding reagent for immunoassay development, molecular diagnostics, affinity purification and other biotin-based applications.

Streptavidin in biotin-based assay workflows

Streptavidin is widely used in life science and diagnostic workflows because of its strong and specific interaction with biotin. In practice, this makes it a useful reagent for assay formats where stable capture, detection or immobilisation of biotinylated molecules is required.

For researchers and assay developers, the quality of Streptavidin can influence workflow parameters such as signal consistency, background binding and reproducibility. This is especially relevant in immunoassays, molecular diagnostics, affinity purification and biotin-based detection systems.

Recombinant Streptavidin from Provis Biolabs

Provis Biolabs offers Streptavidin (#PB-14-10mg / PB-14-100mg) as part of its recombinant bio-reagent portfolio. As an ISO 9001:2015 and ISO 13485 certified manufacturer of recombinant enzymes and bioreagents, Provis Biolabs produces this high-affinity recombinant streptavidin binding protein using Animal-Origin-Free processes to support research, diagnostics and biomanufacturing applications where traceability and batch-to-batch consistency are important.

Provis Biolabs recombinant core Streptavidin is produced in a controlled E. coli expression system and is available as an Animal-Origin-Free recombinant reagent. The core Streptavidin format is an N- and C-terminally truncated variant, developed to support improved homogeneity, solubility, protease resistance and biotin-pocket accessibility compared with native full-length streptavidin.
According to Provis Biolabs, the product offers high binding activity, with a reported specific activity of >17 U/mg protein and ≥95% purity, and is available in both RUO and IVD grades. The product is available in both lyophilized and liquid formats.

Engineered for performance, Provis Biolabs’ core Streptavidin combines high binding activity, high purity and animal-component-free production for consistent, reproducible results across immunoassay, diagnostic and affinity-based workflows.

The product is relevant for laboratories developing or optimising biotin-based assay systems, including workflows where Streptavidin is used for coating, capture, detection or immobilisation. It can support coating workflows for microplates, beads and SPR chips, as well as conjugation workflows involving HRP, fluorophores or magnetic beads.

Application areas

Provis Biolabs recombinant Streptavidin can be used in a range of biotin-based workflows, including:

  • Immunoassay development
  • Molecular diagnostics
  • ELISA-based detection systems
  • Lateral flow assay development
  • Western blot workflows
  • Immunoprecipitation
  • Affinity purification and affinity chromatography
  • Magnetic bead-based separation and purification
  • Biosensor and surface-based assay formats
  • Coating workflows for microplates, beads and SPR chips
  • Conjugation workflows involving HRP, fluorophores and magnetic beads

These application areas align with the Provis Biolabs portfolio of recombinant proteins and enzymes for research, diagnostics and biomanufacturing, including protein purification and diagnostic reagent development.

Why use recombinant, Animal-Origin-Free reagents?

Recombinant and Animal-Origin-Free reagents can help laboratories work with more defined raw materials. In assay development and diagnostic reagent workflows, this can be particularly relevant when consistency, documentation and supply continuity are important considerations.

For laboratories developing biotin-based assays, recombinant Streptavidin offers a defined reagent option for workflows that rely on reproducible biotin-streptavidin binding. The availability of scalable supply can also be relevant for research groups, diagnostic manufacturers and procurement teams looking for dependable sourcing of assay reagents.

Provis Biolabs Streptavidin via Bio-Connect

Provis Biolabs products are available through Bio-Connect for researchers and manufacturers in the Benelux and Europe.

For more information about Provis Biolabs recombinant Streptavidin (#PB-14-10mg / PB-14-100mg) or other recombinant bio-reagents, contact your Bio-Connect account manager or reach out to the Bio-Connect technical support team for product information, documentation, certificates of analysis, availability or bulk enquiries.

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Recombinant Reagents | Enzymes | Growth Factors | Peptides | Custom Peptide Synthesis | RUO, GMP and ISO 13485 | Cell Culture Reagents | IVD | Enzymes for Cell and Gene Therapy

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