Discover the Brilliance of TargetMol’s Fluorescent Dyes
Discover how TargetMol’s advanced fluorescent dyes can provide you with precise, reliable, and intuitive visualization of cellular processes. Empowering every researcher, technician, and procurement specialist in the life sciences.
In cell-based experiments, fluorescent dyes have become powerful tools for revealing cellular structures and functions. Whether it’s changes in membrane potential, mitochondrial status, apoptosis, calcium signaling, or fluctuations in intracellular pH, fluorescence imaging technology provides us with precise and intuitive visualization methods. TargetMol currently offers over 2,600 dyes and dye-related products.
How To Select Dye Products?
Factors
Explanations
Emission Wavelength Compatibility
Compatible with microscope filter settings (e.g., FITC, TRITC, Cy5 channels)
Cell Permeability
Live-cell dyes should exhibit good cell permeability
Cytotoxicity
Non-toxic to cells, suitable for long-term observation
Targeting Specificity
Capable of specific staining of organelles such as the nucleus, mitochondria, endoplasmic reticulum, and lysosomes
Photostability
Resistant to photobleaching, enabling long-term imaging
Cell Membrane Staining
Some dyes, such as Hoechst, are suitable for fixed cells; others, like the Fluo series, are suitable for live cells
Applications
Live Cell Mitochondrial Imaging: Track mitochondrial status using MitoTracker Green FM or Deep Red.
Apoptosis Monitoring: Annexin V-FITC/PI dual staining to distinguish early and late apoptosis.
Calcium Ion Concentration Detection: Monitor calcium signaling with the Fluo-4 AM calcium probe.
pH Change Imaging: Track intracellular/extracellular pH dynamics using pHrodo or SNARF dyes.
Cell Membrane Staining: Stain the cell membrane with dyes such as DiI or FM4-64 to observe endocytosis and related processes.
Nucleic Acid Staining: Identify cell nuclei using Hoechst 33342 dye, suitable for both live and fixed cells.
Li Y, et,al . Hyaluronic acid-coated polypeptide nanogel enhances specific distribution and therapy of tacrolimus in rheumatoid arthritis. J Nanobiotechnology. 2024 Sep 6;22(1):547. IF=10.6
RAW264.7 cells were divided into an LPS-treated group and an untreated group. NG and HA-NG were labeled with Cy5.5 (red). Cell nuclei were stained with DAPI (blue), and the cytoskeleton was stained with SF488-phalloidin (green). The final fluorescence staining results demonstrated the in vitro efficacy of HA-NG/TAC[2].
References
Grimm, J.B., et,al. Caveat fluorophore: an insiders’guide to small-molecule fluorescent labels. Nat Methods 19, 149–158 (2022).
Li Y, et,al. Hyaluronic acid-coated polypeptide nanogel enhances specific distribution and therapy of tacrolimus in rheumatoid arthritis. J Nanobiotechnology. 2024 Sep 6;22(1):547.
Discover the Brilliance of TargetMol’s Fluorescent Dyes
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