Cloud-Clone reagents for organoid research

The rise of organoids as a new paradigm in biomedical research

Organoids have become one of the most dynamic and rapidly growing fields in modern cell biology, disease modeling, and precision medicine. As three-dimensional micro-structures that self-organize in vitro from stem cells or tissue cells, organoids closely mimic the cellular composition, spatial architecture, and key physiological functions of real organs. They serve as a critical bridge between traditional 2D cell culture and animal models, overcoming the limitations of both.

That said, building and validating organoid models depend heavily on precise growth factor supplementation and rigorous biomarker identification. With personalized drug screening and regenerative medicine advancing at pace, standardized, highly sensitive, and comprehensive organoid research reagents have become essential tools for both scientific discovery and clinical translation.

Our partner Cloud-Clone, a global developer of life science research reagents, has built an integrated solution that covers the entire organoid research workflow. From fundamental mechanism studies to drug discovery and biomarker detection, Cloud-Clone provides recombinant proteins, antibodies, ELISA kits, multiplex assay kits, and customized services, enabling scientists worldwide to achieve reliable, reproducible, and high-quality results in organoid research.

Research focus: Organoids, a central pillar of precision medicine

Organoids are derived from three main cell sources: pluripotent stem cells (ESC/iPSC), adult stem cells (ASC), and patient-derived tumor tissues. Organoids have now been successfully established for nearly all major human organ systems, including the brain, liver, kidney, intestine, lung, heart, stomach, and a wide range of tumor types.

Key organoid types and their applications

1. Brain Organoids
   Model human brain development and neurological disorders. Key markers include PAX6, SOX2, TBR2, TUBb3, SATB2, NKX2.1, GFAP, OLIG2, and MBP.
   
2. Liver Organoids
   Simulate liver development, metabolism, and disease. Key markers include EPCAM, CK19, AFP, ALB, HNF4α, CYP3A4, SOX9, CK7, GFAP, Des, and CD68.
   
3. Kidney Organoids
   Recapitulate nephrogenesis and kidney disease. Key markers include WT1, NPHS1, PODXL, CUBN, ECAD, GATA3, UMOD, CD31, AQP1, PAX2, and PAX8.
   
4. Intestinal Organoids
   Model intestinal homeostasis, infection, and inflammation. Key markers include LGR5, OLFM4, LYZ, DEFA5/6, MUC2, TFF3, CHGA, SYP, CDX2, and ALPI.
   
5. Lung Organoids
   Simulate lung development and respiratory disease. Key markers include SOX2, SOX9, TP63, CK5, FOXJ1, MUC5AC, SFTPC, SFTPB, LAMP3, ABCA3, HOPX, AQP5, and PDPN.
   
6. Cardiac Organoids
   Model heart development and cardiac injury. Key markers include TNNT2, MYL2, MYL7, ISL1, FOXC1, TBX3, CD31, CDH5, WT1, MYH6, NKX2-5, and cTnI.
   
7. Tumor Organoids
   Patient-derived tumor organoids (PDOs) are powerful platforms for drug screening and personalized therapy. Key markers include CDX2, CK20, LGR5, and Ki-67 (colorectal cancer); ERa, PGR, HER2, and EGFR (breast cancer); TTF1, NapsinA, p40, and CK5/6 (lung cancer); AFP, GPC3, CK19, and EpCAM (liver cancer); PAX8, PAX2, CA9, CD10, and VIM (renal cancer).

Organoid dysregulation and disease

Abnormalities in organoid development and validation are linked to a broad range of research challenges, including cancer research (colorectal, breast, gastric, lung, liver, and renal cancers), inflammatory and ischemic injury (liver injury, kidney disease, myocardial ischemia-reperfusion injury), neurological and ophthalmic disorders (Alzheimer’s disease, brain developmental disorders), and regenerative medicine (tissue repair and organ replacement).

Key molecules such as LGR5, SOX2, PAX6, ALB, WT1, TNNT2, CDX2, and EpCAM have become major targets in both organoid biology research and therapeutic development.

Cloud-Clone: A Full-Spectrum Partner for Organoid Research

Cloud-Clone offers five core products and services—recombinant proteins, antibodies, ELISA kits, multiplex assay kits, and customized services—providing essential reagent support for every stage of organoid research, from model establishment and differentiation validation to disease modeling, drug screening, and biomarker quantification.

Core Advantages

1. Comprehensive portfolio: one-stop product matrix
   Cloud-Clone covers raw materials for the entire organoid experimental cycle: 800+ self-developed recombinant cytokines (including GMP-grade scaled products), organoid tissue marker-specific antibodies, supporting ELISA quantitative detection kits, and multiplex assay kits. This one-stop solution covers the complete workflow of organoid induction culture, morphological identification, and target protein quantification. No need to source from multiple suppliers.
   
2. High quality: built for stringent organoid culture requirements
   Recombinant cytokines with high biological activity, ultra-low endotoxin levels, and excellent batch-to-batch stability; antibodies with strong specificity and low cross-reactivity; ELISA kits with high sensitivity and good reproducibility. These products are designed to meet the high standards of organoid stem cell induction and differentiation, phenotypic identification, and precise target quantification.
   
3. Strict quality control backed by triple authority certifications
   ISO9001/ISO13485/ISO14001 triple system certifications, with standardized control throughout the entire process, from raw material traceability and production preparation to finished product release, ensuring controllable batch stability.
   
4. Customized services that meet individualized research needs
   End-to-end customization services, from target design to final product delivery, flexibly adapted to a wide range of innovative research projects.

Key Organoid Targets Supported by Cloud-Clone

1. Recombinant proteins (growth factors and cytokines)

Cloud-Clone offers a comprehensive range of high-purity recombinant proteins, covering key growth factors and cytokines such as EGF, FGF2, FGF4, FGF7, FGF10, BMP4, NOG, RSPO1, WNT3A, HGF, GDNF, BDNF, NT3, TGFb1, IGF1, VEGF, LIF, and ACVA. These proteins are designed for in vitro organoid establishment, differentiation, functional validation, and drug screening. Each batch undergoes rigorous quality control to ensure consistent biological activity and reproducibility.

2. Organoid-specific antibodies

Cloud-Clone offers monoclonal and polyclonal antibodies targeting organoid markers, with high specificity, minimal cross-reactivity, and validated performance across multiple applications. These antibodies are widely used in Western blot (WB), immunohistochemistry (IHC), immunofluorescence (IF), and flow cytometry, enabling precise detection of organoid differentiation and maturation in tissues and cells, and supporting disease mechanism studies and biomarker discovery.

3. High-sensitivity ELISA kits

Cloud-Clone’s ELISA kits are one of the company’s flagship product lines, covering key organoid biomarkers including LGR5, OLFM4, SOX2, PAX6, ALB, AFP, WT1, NPHS1, TNNT2, CDX2, EpCAM, and CK19. They are compatible with multiple sample types (cell supernatant, tissue homogenate, serum, plasma) and are available in both standard colorimetric and high-sensitivity chemiluminescent formats, making them ideal for large-scale studies and clinical biomarker analysis.

4. Multiplex assay kits

Cloud-Clone offers multiplex assay kits for simultaneous quantification of multiple organoid-related markers and cytokines, suitable for parallel analysis of multiple targets in complex samples. This product line is particularly well-suited for research scenarios requiring high-throughput, multi-indicator detection, such as dynamic monitoring of organoid differentiation, multi-target response assessment in drug screening, and systematic analysis of disease models.

5. Customized organoid research solutions

To support advanced and innovative research projects, Cloud-Clone offers end-to-end customization services, from target design to final product delivery. Customized services include the development of organoid-related recombinant proteins, antibodies, ELISA kits, and multiplex assay kits, flexibly meeting individualized research needs.

Cloud-Clone has developed a comprehensive set of reagents for organoid research. Selected representative targets and core product numbers include:

For the complete organoid-related target overview, including specific markers, growth factors and corresponding Core Product Numbers, please see the table after the References.

Driving innovation in organoid research and precision medicine

As cell biology and precision medicine continue to advance, organoids are emerging as a critical platform for disease modeling and therapeutic intervention. By providing high-quality recombinant proteins, antibodies, ELISA kits, multiplex assay kits, and customized services, Cloud-Clone equips researchers worldwide with reliable experimental tools that accelerate discoveries in organoid biology and support the development of innovative therapies.

From fundamental stem cell research to next-generation drug development, Cloud-Clone remains committed to delivering high-quality, innovative, and comprehensive reagent solutions, empowering scientists globally and accelerating the translation of scientific breakthroughs into clinical applications.

Working with organoids, patient-derived models or biomarker analysis? Bio-Connect can help you find the right Cloud-Clone reagents for your research in the Benelux and Europe. Whether you are establishing a new model, validating organoid markers or selecting reagents for biomarker detection, our team is happy to think along and point you towards suitable products.

References

[1] Sato T, Vries RG, Snippert HJ, et al. Single Lgr5 stem cells build crypt-villus structures in vitro without a mesenchymal niche. Nature. 2009;459(7244):262-265.
[2] Lancaster MA, Renner M, Martin CA, et al. Cerebral organoids model human brain development and microcephaly. Nature. 2013;501(7467):373-379.
[3] Wang S, Wang X, Tan Z, et al. Human ESC-derived expandable hepatic organoids enable therapeutic liver repopulation and pathophysiological modeling of alcoholic liver injury. Cell Res. 2019;29(12):1009-1026.
[4] Takasato M, Er PX, Chiu HS, et al. Kidney organoids from human iPS cells contain multiple lineages and model human nephrogenesis. Nature. 2015;526(7574):564-568.
[5] Yang L, Wang X, Zhou X, et al. A tunable human intestinal organoid system achieves controlled balance between self-renewal and differentiation. Nat Commun. 2025;16(1):315.
[6] Chen YW, Huang SX, de Carvalho ALRT, et al. A three-dimensional model of human lung development and disease from pluripotent stem cells. Nat Cell Biol. 2017;19(5):542-549.
[7] Volmert B, Kiselev A, Juhong A, et al. A patterned human primitive heart organoid model generated by pluripotent stem cell self-organization. Nat Commun. 2023;14(1):8245.
[8] Yu BM, Lee SD, Hwang BR, et al. Application of an organoid-based model to explore Helicobacter pylori-human gastric epithelium interaction in vitro. Front Cell Infect Microbiol. 2025;15:1572244.
[9] Crespo M, Vilar E, Tsai SY, et al. Colonic organoids derived from human induced pluripotent stem cells for modeling colorectal cancer and drug testing. Nat Med. 2017;23(7):878-884.
[10] Chen P, Zhang X, Ding R, et al. Patient-Derived Organoids Can Guide Personalized-Therapies for Patients with Advanced Breast Cancer. Adv Sci (Weinh). 2021;8(22):e2101176.
[11] Kim M, Mun H, Sung CO, et al. Patient-derived lung cancer organoids as in vitro cancer models for therapeutic screening. Nat Commun. 2019;10(1):3991.
[12] Seidlitz T, Merker SR, Rothe A, et al. Human gastric cancer modelling using organoids. Gut. 2019;68(2):207-217.
[13] Yang H, Cheng J, Zhuang H, et al. Pharmacogenomic profiling of intra-tumor heterogeneity using a large organoid biobank of liver cancer. Cancer Cell. 2024;42(4):535-551.e8.
[14] Li Z, Xu H, Yu L, et al. Patient-derived renal cell carcinoma organoids for personalized cancer therapy. Clin Transl Med. 2022;12(7):e970.

Complete Cloud-Clone organoid-related target overview

The table below provides a complete overview of Cloud-Clone organoid-related targets, including organoid-specific markers, growth factors, and corresponding Core Product Numbers. It is intended as a practical reference for researchers selecting reagents for organoid establishment, characterization, differentiation studies, and biomarker analysis.