11 June 2024

SCICONS dsRNA Antibodies for Virology Research

SCICONS™ anti-dsRNA antibodies have been cited in more than 400 studies for applications such as detecting whether an unknown pathogen is bacterial or viral, enabling viral life cycle research and studying anti-viral responses.

Researchers are increasingly relying on anti-dsRNA antibodies as crucial tools in their studies due to the pivotal role of double-stranded RNA (dsRNA) in cellular processes like viral replication. To support dsRNA-focused research in fields like virology, the SCICONS™ line of anti-dsRNA antibodies, kits, and controls were developed and have been highly cited since their initial discovery in 1990.
The SCICONS antibodies include:

J2 monoclonal antibody (10010200 and 10010500), which is the clone most often used to detect dsRNA intermediates of a wide range of viruses in cells and tissues, for applications including IHC, IF, Western blot, ELISA and electron microscopy.

J5 monoclonal antibody (10040200 and 10040500), which is comparable in dsRNA affinity and specificity to the J2 clone, but has a different isotype, making it a flexible tool for simultaneous detection of dsRNA with other markers in IF and more.

K1 monoclonal antibody (10040200 and 10040500), which recognizes dsRNA with similar affinity to the J2 antibody. It can be used for the histological and cytological detection of dsRNA in cells and tissues as an alternative to J2. This clone is also the best option for detecting poly I:C.

K2 monoclonal antibody (10030005 and 10030010), which has an IgM isotype and is typically used in specialized applications where an anti-dsRNA antibody with an isotype other than IgG is required.

Additionally, SCICONS anti-dsRNA kits are available, with the reagents needed to perform a sandwich ELISA and detect dsRNA in your samples efficiently. Key offerings include J2-based kits (10613002 and 10613005) and K1-based kits (10623002 and 10623005).

Virus Research with SCICONS Antibodies

SCICONS antibodies enable precise detection and characterization of dsRNA, deepening our understanding of RNA biology. Particularly valuable in viral research, they help investigators uncover insights into RNA metabolism, pathogen diagnostics, and viral life cycles.

The J2, J5, K1, and K2 monoclonal antibody clones that comprise the SCICONS product range are used to detect dsRNA intermediates of a diverse range of viruses, including chikungunya, dengue (see Figure 1), hepatitis, rabies, rhinovirus, polio, swine fever and more.

Figure 1.  Immunofluorescence microscopy using J2 antibody reveals dsRNA (labeled in red), a marker for the viral replication complex, in HuH-7 cells infected with Dengue virus. Cellular DNA is labelled with DAPI (blue). Figure taken from Anwar et al. (2011) PLoS One 6:e23246.

The SCICONS antibodies’ high specificity, affinity for a range of synthetic double-stranded polyribonucleotides, and lack of reaction with DNA and single-stranded RNA have been extensively characterized and make them powerful tools for a variety of research.

Recombinant Anti-dsRNA Antibodies

For even more specificity and biological definition, the team at Absolute Antibody engineered the J2 clone into recombinant, species-swapped antibodies. This provides researchers with additional options for anti-dsRNA detection and quantitation. Formats include the J2 clone in mouse IgG2a with the Fc Silent™ mutation (Ab01299-2.3) to abrogate Fc region binding, reducing background noise. Other species and isotypes are available for added experimental possibility or explore Absolute Antibody’s custom antibody engineering services to craft the perfect antibody reagent for your research.


The SCICONS™ anti-dsRNA antibody line has emerged as a crucial asset for researchers exploring diverse scientific realms, particularly within virology. SCICONS antibodies continue to contribute significantly to cutting-edge research, providing researchers with powerful tools to probe the intricate landscape of infectious diseases and cellular processes associated with double-stranded RNA.

SCICONS dsRNA Antibodies for Virology Research

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