INDIGlo Luciferase Detection Reagent kit is designed for the quantification of firefly and beetle luciferase expression in mammalian cells.
The INDIGlo Luciferase Detection Reagent kit is formulated as a 2x-concentrated reagent which contains both the luciferase detection reagent (LDR) and dilution buffer and can be used for both homogenous and non-homogenous assay applications. With a signal duration of greater than 2 hour and with a less than 10% fluctuation in signal, the highly sensitive and stable luminescence signal of INDIGlo is ideal for the cost-effective processing of cell culture plates ranging from 96 to 1536-well format.
This product will be of particular interest to customers that develop their own cell-based firefly luciferase reporter assay.
INDIGlo luciferase detection reagent performance
INDIGlo LDR performance from both Homogenous and Non-Homogenous assays. Dose-response analyses of Human AhR (INDIGO #IB06001, 96-well assay) was performed using the reference agonist MeBIO. AhR reporter cells were plated across two white assay plates, MeBIO treatment media were added, and the assay plates incubated for 24 hr. Plates were then processed with luciferase detection reagents in either a.) a homogenous or b.) a non-homogenous assay format. Resulting assay metrics are also shown from using another extended-glo detection reagent (Vendor X).INDIGlo LDR potency is stable following repeated freeze and thaw cycles. INDIGlo LDR was frozen at -20°C and thawed either 1, 2 or 3 times, then used to quantify luciferase activity from HEK-293:luc cells cultured in a white 96-well assay plate. No loss of reagent potency is observed between these conditions.
INDIGlo luciferase detection reagent kit
10 mL each of 2x-concentrated INDIGlo LDR and Dilution Buffer (LDR-10)
25 mL each of 2x-concentrated INDIGlo LDR and Dilution Buffer (LDR-25)
50 mL each of 2x-concentrated INDIGlo LDR and Dilution Buffer (LDR-50)
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