Linking NAD+ Signaling & Cancer

Many and various NAD+-dependent signaling pathways regulate fundamental processes such as transcription, DNA repair, cell cycle progression, apoptosis, proliferation, survival and metabolism, which are linked to cancer development. NAD+-dependent signaling reactions involve the degradation of NAD, therefore permanent nucleotide resynthesis through different biosynthetic pathways is crucial for incessant cancer cell proliferation. Targeting the NAD metabolism, its synthesis and signaling pathways (PARPs, SIRTs, MARTs, CD38) show several effects on cancer cells and are therefore new and promising concepts for cancer treatment.

Literature References: Nampt: Linking NAD biology, metabolism and cancer: A. Garten, et al.; Trends Endocrinol. Metab. 20, 130 (2009) • The blockade of immune checkpoints in cancer immunotherapy: D.M. Pardoll; Nat. Rev. Canc. 12, 253 (2012)

Nicotinamide phosphoribosyltransferase (Nampt)

Nampt (visfatin; PBEF) converts nicotinamide (Nam), that arises endogenously from NAD+-dependent signaling processes, to nicotinamide mononucleotide (NMN), which is further converted to NAD+ through NMNATs. Nampt has recently drawn much attention in several fields, including NAD biology, metabolism, type 2 diabetes, inflammation and cancer. It regulates the activity of NAD-consuming enzymes such as sirtuins and influences a variety of metabolic and stress responses.

Nampt (visfatin/PBEF) ELISA kits, proteins, antibodies and inhibitors

Cat. No.DescriptionAmountBrand
AG-45A-0006YEKNampt (Visfatin/PBEF) (human) ELISA Kit96 wellsAdipoGen
AG-45A-0008YEKNampt (Visfatin/PBEF) (human) (IntraCellular) ELISA Kit96 wellsAdipoGen
AG-45A-0007YEKNampt (Visfatin/PBEF) (mouse/rat) Dual ELISA Kit96 wellsAdipoGen
AG-40A-0031YNampt (Visfatin/PBEF) (human) (rec.)3 x 10 ugAdipoGen
AG-40A-0018Nampt (Visfatin/PBEF) (human) (rec.) (His)50 ugAdipoGen
AG-40A-0056YNampt (Visfatin/PBEF) (mouse) (rec.)3 x 10 ugAdipoGen
AG-40A-0017Nampt (Visfatin/PBEF) (mouse) (rec.) (His)50 ugAdipoGen
AG-40A-0058Nampt (Visfatin/PBEF) (rat) (rec.)10 ugAdipoGen
AG-25A-0025anti-Nampt (Visfatin/PBEF) (human), pAb100 ugAdipoGen
AG-25A-0025Banti-Nampt (Visfatin/PBEF) (human), pAb (Biotin)50 ugAdipoGen
AG-25A-0027anti-Nampt (Visfatin/PBEF) (human), pAb (CT)100 ugAdipoGen
AG-25A-0026anti-Nampt (Visfatin/PBEF) (human), pAb (NT)100 ugAdipoGen
AG-20A-0034anti-Nampt (Visfatin/PBEF), mAb (OMNI379)50 ugAdipoGen
AG-20A-0034Banti-Nampt (Visfatin/PBEF), mAb (OMNI379) (Biotin)50 ugAdipoGen
AG-CR1-0064CHS-828 [200484-11-3]5 mgAdipoGen
AG-CR1-0011FK-866 [658084-64-1]1 mgAdipoGen
AG-40T-0091NAD Kinase (human) (rec.) (His) (highly active)50 ugAdipoGen
AG-40T-0090NAD Kinase (catalytic domain) (human) (rec.) (His) (highly active)50 ugAdipoGen
AG-40T-0092NMNAT1 (human) (rec.) (His) (highly active)3 x 50 ugAdipoGen
AG-40A-0153TNMNAT2 (human) (rec.) (His)3 x 10 ugAdipoGen
AG-40T-0093NMNAT3 (human) (rec.) (His) (highly active)3 x 50 ugAdipoGen

Sirtuins (SIRTs) and cancer

NAD+-dependent deacetylation by sirtuins results in the modulation of target protein activity, stability or localization. Sirtuins establish a direct link between metabolic and bioenergetic homeostasis and gene regulation. They were shown to be involved in the development of diabetes, obesity, metabolic syndrome and cancer.

Sirtuin ELISA kits, proteins, antibodies and modulators

Cat. No.DescriptionAmountBrand
AG-45A-0029YEKSirtuin 1 (human) (IntraCellular) ELISA Kit96 wellsAdipoGen
AG-25A-0082anti-Sirtuin 1 (human), pAb100 ugAdipoGen
AG-20A-0076anti-Sirtuin 2 (human), mAb (S2R233-1)50 ugAdipoGen
AG-CR1-3511AK-7 [420831-40-9]5 mgAdipoGen
AG-CN2-0008Hyperforin . DCHA [238074-03-8]500 ugAdipoGen
AG-CN2-0086Piceatannol [10083-24-6]1 mgAdipoGen
AG-CN2-0033Resveratrol [501-36-0]50 mgAdipoGen
AG-CN2-0036Resveratrol (synthetic) [501-36-0]50 mgAdipoGen
AG-CR1-0055Sirtinol [410536-97-9]1 mgAdipoGen
AG-CR1-0088Splitomicin [5690-03-9]1 mgAdipoGen
AG-CR1-3575Suramin . hexasodium salt [129-46-4]50 mgAdipoGen

Poly(ADP ribose) polymerases (PARPs)

In the context of NAD+-dependent signaling in tumorigenesis, polyADP ribosylation is the most promising target for cancer therapy. Poly(ADP ribose) polymerases (PARPs) contribute to cell transformation by regulating the activity of key transcription factors that are involved in cell cycle regulation and apoptosis. Given that the activity of these transcription factors is significantly affected by poly(ADP ribose) formation, NAD+ consumption by PARPs seems to be central in regulating transcriptional events that are relevant to neoplastic transformation.

PARP proteins, antibodies and inhibitors

Cat. No.DescriptionAmountBrand
AG-40T-0011PARP-1 [ARTD1] (human) (rec.) (His) (high purity)10 ugAdipoGen
AG-40T-0010PARP-1 [ARTD1] (catalytic domain) (human) (rec.) (His)20 ugAdipoGen
AG-40T-0015PARP-1 [ARTD1] (E998K Mutant) (human) (rec.) Control10 ugAdipoGen
AG-40T-0012PARP-2 [ARTD2] (mouse) (rec.) (His) (high purity)10 ugAdipoGen
AG-40T-0013PARP-3 [ARTD3] (human) (rec.) (His) (high purity)10 ugAdipoGen
AG-40T-0022PARG (human) (rec.) (His) (highly active)2 ugAdipoGen
AG-25T-0002anti-PARP-1 [ARTD1] (human), pAb100 ulAdipoGen
AG-25T-0001anti-PARP-1 [ARTD1] (mouse), pAb100 ulAdipoGen
AG-25T-0003anti-PARP-2 [ARTD2] (mouse), pAb100 ulAdipoGen
AG-20T-0001anti-Poly(ADP-ribose) [PAR], mAb (10H)50 ugAdipoGen
AG-CR1-00241,5-Isoquinolinediol [5154-02-9]5 mgAdipoGen
AG-CR1-0100PJ-34 [344458-15-7]1 mgAdipoGen
AG-CR1-0037DPQ [129075-73-6]1 mgAdipoGen

CD38 antibodies

CD38 is the major NAD glycohydrolase and ADP-ribosyl cyclase, producing ADPR, cyclic ADPR and NAADP. CD38 has been identified as a negative prognostic marker in chronic lymphocytic leukaemia (CLL).

Cat. No.DescriptionAmountBrand
ANC-187-020anti-CD38 (human), mAb (AT1)100 ugAncell
ANC-187-820anti-CD38 (human), mAb (AT1) (preservative free)100 ugAncell
ANC-187-030anti-CD38 (human), mAb (AT1) (Biotin)100 ugAncell
ANC-187-040anti-CD38 (human), mAb (AT1) (FITC)120 testsAncell
ANC-187-050anti-CD38 (human), mAb (AT1) (R-PE)120 testsAncell

Website: AdipoGen Life Sciences

Related to:

Brands: AdipoGen Life Sciences, Ancell Corporation
Product groups: Antibodies, Assays, Chemicals, Proteins / Signaling Molecules