22 October 2014

LymphoTrack® Assays

Invivoscribe’s LymphoTrack® Assays are used to identify and track clonal lymphocyte populations. These RUO assays, developed for use with the leading Next-Generation Sequencing (NGS) platforms, include optimized multiplex PCR master mixes with primers incorporating both platform specific adapters, and specimen tracking Seq ID tags for single step workflow. They also include a comprehensive bioinformatics package, enabling you to identify the DNA sequence, clonal prevalence, and V-J family identity for each gene rearrangement.

Key benefits of LymphoTrack® Assays

• One-step PCR for streamlined workflow
• Identical workflow for clonal detection and MRD analysis
• Multiple indices for simultaneous testing of multiple samples with multiple assays
• Bioinformatics software for simple, automated visualization of data and analysis
• Unparalleled sensitivity of clonality detection

LymphoTrack^® RUO assays are offered as kits for either the Illumina MiSeq^® or Ion PGM™ platforms. See below for platform specific information.

LymphoTrack® Assays for the Illumina MiSeq®

The LymphoTrack MiSeq master mixes are designed with 24 TruSeq indices and adapters for the Illumina MiSeq instrument, allowing one-step PCR, pooling of amplicons, and loading onto the flowcell.

• LymphoTrack IGH Assay – MiSeq
• LymphoTrack IGK Assay – MiSeq
• LymphoTrack TRG Assay – MiSeq
• LymphoTrack IGH + TRG Assay – MiSeq
• LymphoTrack IGH Somatic Hypermutation Assay – MiSeq

LymphoTrack® Assays for the Ion PGM®

The LymphoTrack PGM master mixes are designed with 12 Ion Xpress™ indices and adapters for the Ion PGM instrument, allowing one-step PCR, pooling of amplicons, and loading onto the chip.

• LymphoTrack IGH Assay – PGM
• LymphoTrack IGK Assay – PGM
• LymphoTrack TRG Assay – PGM
• LymphoTrack IGH + TRG Assay – PGM
• LymphoTrack IGH Somatic Hypermutation Assay – PGM

Workflow: A single-step PCR library preparation is followed by standard MiSeq or PGM sequencing. The proprietary LymphoTrack Bioinformatics Software (included in the assay kit) directly converts NGS data output to a format that allows you to visualize and analyze the prevalence or frequency distribution of the gene rearrangements and to identify specific DNA sequences. The software facilitates interpretation and subsequent tracking of clonal populations. With this state-of-the-art assay, the gene rearrangement status of each of the several million lymphocytes in blood or tissue samples can be identified and characterized.

References

• Stamatopoulos B, Timbs A, Bruce D, et al. Targeted deep sequencing reveals clinically relevant subclonal IgHV rearrangements in chronic lymphocytic leukemia. Leukemia. 2017;31(4):837-845.
• Shin S, Hwang IS, Kim J, Lee KA, Lee ST, Choi JR. Detection of immunoglobulin heavy chain gene clonality by next-generation sequencing for minimal residual disease monitoring in b-lymphoblastic leukemia. Ann Lab Med. 2017;37(4):331-335.
• Lamarche C, Orio J, Georges-Tobar V, et al. Clinical-scale rapid autologous bk virus-specific t cell line generation from kidney transplant recipients with active viremia for adoptive immunotherapy. Transplantation. 2017;101(11):2713-2721.
• Levy-Mendelovich S, Lev A, Rechavi E, et al. T and B cell clonal expansion in Ras-associated lymphoproliferative disease (Rald) as revealed by next-generation sequencing. Clin Exp Immunol. 2017;189(3):310-317.