Specialized in the design, development and manufacturing of ready-to-use TR-FRET assay kits

BioAuxilium Research is a Canadian biotechnology tool company focused on developing and manufacturing cutting-edge cell-based immunoassays based on their enhanced proprietary TR-FRET technology platform, THUNDER™. Founded by experienced assay developers from PerkinElmer with a track record of launching commercially successful assay kits and reagents, BioAuxilium offers top quality, highly validated yet affordable TR-FRET assay kits designed to accelerate all stages of drug discovery. Their products are backed by comprehensive and transparent validation data, along with outstanding technical support and fast delivery. Skeptical? Put them to test!

BioAuxilium also offers a range of custom services at an outstanding value, including antibody labeling to their proprietary donor and acceptor fluorophores, best antibody pair analysis, assay development, ELISA conversion, assay kit development, and compound profiling. Their turnaround times are the shortest in the industry.

Visit BioAuxilium’s booth at SLAS Europe 2023 in Brussels on May 24 and 25.

Why choose TR-FRET assays from BioAuxilium?

Better components

  • Optimal donor/acceptor fluorophores for exceptional TR-FRET signal
  • Superior antibodies specifically selected and validated for TR-FRET
  • Optimized lysis and assay buffers

Rigorous validation

  • All kits are highly validated on relevant cellular samples
  • Technical datasheets contain all validation data


  • Kits are developed, validated, and manufactured in-house
  • New lots are measured against existing lots to ensure lot-to-lot consistency


  • A superior, much cost-effective alternative to current TR-FRET kits
  • Total flexibility: kits available in 100, 500, 2500, 5000, and 10000 assay points sizes, along with any bulk size


Learn more about the THUNDER™ TR-FRET platform in just 3 minutes.

How it works?

In a typical sandwich assay configuration, one antibody is labeled with the Europium chelate donor and the second antibody is labeled with the far-red acceptor fluorophore. The binding of the two labeled-antibodies to the target protein in the cell lysate or supernatant brings donor and acceptor molecules into close proximity. Upon excitation of the Europium chelate at 320 or 340 nm, energy is transferred to the far-red fluorophore, which in turn emits a long-lived fluorescence signal at 665 nm. The intensity of light emission at 665 nm is proportional to the level of protein in the sample.

Best sellers (endogenous kinases):

THUNDER Phospho-AKT pan (S473) TR-FRET Cell Signaling Assay Kit (KIT-AKTS473P)
THUNDER Extreme Phospho-ERK1/2 (T202/Y204) TR-FRET Cell Signaling Assay Kit (KIT-XTM-ERKP)
THUNDER Phospho-STAT5 (Y694/Y699) TR-FRET Cell Signaling Assay Kit (KIT-STAT5P)
THUNDER Phospho-SLP-76 (S376) TR-FRET Cell Signaling Assay Kit (KIT-SLP76P)
THUNDER Phospho-EGFR (Y1068) TR-FRET Cell Signaling Assay Kit (KIT-EGFRP)

Best sellers (human cytokines):

THUNDER Human IFNgamma TR-FRET Biomarker Assay Kit (KIT-IFNG)
THUNDER Human IL-2 TR-FRET Biomarker Assay Kit (KIT-IL2)
THUNDER Human TNFalpha TR-FRET Biomarker Assay Kit (KIT-TNFA)
THUNDER Human IL-1beta TR-FRET Biomarker Assay Kit (KIT-IL1B)
THUNDER Human IL-12/IL-23 p40 TR-FRET Biomarker Assay Kit (KIT-IL12)

THUNDER™ TR-FRET Biomarker Assay Kits

Mouse IFNβHuman IL-8/CXCL8Human TNFα
Human IFNαHuman IL-10Human MMP-3
Human IFNγHuman IL-11Human PD-L1
Mouse IFNγHuman IL-12/IL-23 p40Human TNFα
Human IL-1αHuman IL-12 p70Mouse TNFα
Mouse IL-1αHuman IL-17AHuman GM-CSF
Human IL-1βHuman IL-18Human COL1A1
Mouse IL-1βHuman FibronectinHuman VEGF
Human IL-2Human InsulinMouse VEGF
Mouse IL-2Human CCL2/MCP1Human IgG (Fc)
Human IL-6Mouse CCL2/MCP1
Mouse IL-6Human CCL4/MIP-1β

Bio-Connect Diagnostics supplies all their products in the Benelux. If you have any questions, please contact the helpdesk.

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